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PLOS One logoLink to PLOS One
. 2025 Dec 18;20(12):e0338202. doi: 10.1371/journal.pone.0338202

Assessing the clinical relevance of point-of-care ultrasound for hospitalists: Influence on clinical reasoning and decision-making

Maira Dias Souza 1,*, Hassan Rahhal 1,2, Iolanda F L C Tibério 3
Editor: Biswabandhu Jana4
PMCID: PMC12714290  PMID: 41411244

Abstract

Introduction

Point-of-care ultrasonography (POCUS) is increasingly recognized in internal medicine, yet its influence on hospitalists’ clinical reasoning remains underexplored.

Objectives

This study aimed to evaluate the influence of POCUS on diagnostic hypotheses, management strategies, and confidence levels among hospitalists in a transitional care unit with limited access to advanced imaging.

Methods

Prospective descriptive study in a transitional care unit enrolling 19 hospitalists. During routine care, clinicians used POCUS at their discretion and, immediately afterward, completed a structured form capturing the clinical question, pre- and post-POCUS diagnostic hypotheses, confidence levels, and management decisions. Per-encounter changes in these parameters were summarized descriptively.

Results

POCUS led to a change in the primary diagnostic hypothesis in 37% (38/104) of clinical encounters and altered management in 39% (41/104) of cases. Diagnostic confidence increased in 46% (48/104) of instances. Even when the primary hypothesis remained unchanged (62% − 66/104), POCUS strengthened diagnostic confidence in 36% (24/66) of these cases and aided in ruling out alternative diagnoses in 62% (41/66). Notably, POCUS had no discernible influence in only 13% (14/104) of cases.

Conclusion

This study suggests that POCUS may influence clinical reasoning and decision-making among hospitalists in a setting with limited imaging resources. POCUS frequently led to changes in diagnostic hypotheses and management plans, and often increased diagnostic confidence, even when the initial hypothesis was maintained. These findings suggest that POCUS may play a supportive role in bedside assessment and patient management in internal medicine, underscoring the need for further research and structured training before broader integration.

Introduction

Point-of-care ultrasonography (POCUS) has been widely adopted across various medical specialties as a rapid, bedside imaging tool that enhances diagnostic accuracy and clinical decision-making [15]. Its use is well established in critical care settings, where it has demonstrated benefits, such as reducing diagnostic uncertainty, improving patient management, and improving first-attempt procedural success ratio [612]. Numerous medical societies endorse POCUS for these applications, with evidence linking its use to improved patient outcomes, reduced hospital stays, and lower healthcare costs [1330].

Although initial research of its applications were mainly conducted in emergency and intensive care settings [3134], POCUS is gaining interest in internal medicine, particularly among hospitalists [3554]. Aligning POCUS to classical bedside evaluation has the potential to enhance the assessment of pleural effusion [5557], pneumothorax [5557], reduced ejection fraction and heart failure [55,56,58], deep vein thrombosis [59], and volume status [60,61]. However, research on its clinical application in internal medicine remains limited [6266]. Its impact on key outcomes such as length of stay, readmission rates, and mortality in general inpatient settings remains underexplored [64,6769].

As evidence indicates POCUS affects diagnostic accuracy, it is reasonable to hypothesize it may influence clinical reasoning and decision-making by refining diagnostic probabilities and reducing uncertainty in challenging cases. Although studies suggest that POCUS leads to unexpected diagnoses and management changes among hospitalists, its direct influence on clinical reasoning remains understudied [64,7077].

This study aims to evaluate the influence of POCUS on hospitalists’ clinical reasoning and decision-making by analyzing changes in diagnostic hypotheses, management strategies, and physician confidence levels.

Materials and methods

Study design

This is a descriptive prospective study. Research protocol was approved by our Research Ethics Committee [approval number: 6.292.577; CAAE 73039523.3.0000.0068]. Written informed consent was obtained from all participants. Data collection was conducted between October 11, 2024, and January 21, 2025, at the Transitional Care Unit of the Instituto Perdizes (Perdizes Institute/IPer). IPer is an institute of the Hospital das Clínicas, Faculty of Medicine, University of São Paulo, a hospital complex with 2,590 beds. The Transitional Care Unit at IPer has 70 beds and serves adult patients who require a transition from acute care to post-acute care. Its primary goals are to enhance patients’ rehabilitation, educate caregivers on complex care needs, and ensure a safe transition to outpatient care. The medical team consists solely of hospitalists specialized in general internal medicine, with no on-site subspecialists available. Imaging resources are limited to conventional radiography, and advanced imaging requires inter-hospital transfer. The average patient age admitted to IPer is 58 years old. The most frequent reasons for admission are stroke, traumatic brain injury, spinal cord injury, surgical site infections, infective endocarditis, post-valve replacement surgery, and heart failure.

Participants and procedures

All hospitalists were invited to participate. After signing the written Informed Consent Form, participants completed a form collecting personal data (Fig 1), including age, gender, education, previous POCUS experience, and any prior formal POCUS training (S1 Appendix A).

Fig 1. Study procedures flowchart.

Fig 1

Hospitalists who agreed to participate signed an informed consent form (ICF) and completed a personal data form. They then attended a brief POCUS orientation. During routine care, POCUS was used at clinicians’ discretion. Immediately after each use, participants completed a structured encounter form documenting the clinical question, pre- and post-POCUS diagnostic hypotheses, confidence levels, and management decisions. At the end of the study, participants completed a perceptions/satisfaction survey.

Following the initial assessment, all participants underwent an introductory educational session on POCUS (Fig 1), which covered basic applications in clinical practice, equipment use and maintenance, transducer types, image optimization, and interpretation of images from lungs, pleura, heart, abdomen, and vessels. This session was not based on a standardized protocol; rather, it provided a broad overview of commonly relevant organ systems to internal medicine hospitalists. The emphasis was on introducing participants to the potential clinical applications of POCUS rather than on training them to follow a fixed, structured protocol.

Throughout the study, participants had access to a portable ultrasound machine (Mindray DC-80A/2022) and used it as needed in clinical practice. Examinations were performed at the discretion of each physician according to the clinical question, without following a predefined or standardized protocol. Written Informed Consent was obtained from all patients who underwent POCUS examinations. Immediately after each use, participants completed a detailed form (Fig 1) documenting the reason for using POCUS, the initial diagnostic hypothesis, planned actions, the ultrasound modalities used—chosen from seven options (cardiac, pulmonary, abdominal, inferior vena cava, femoral and popliteal veins, bladder, and other), with the possibility of selecting multiple modalities per form—, the estimated duration of the exam (self-reported), the perceived quality of the images obtained (self-reported), findings, final diagnostic hypothesis, and subsequent clinical decisions (S2 Appendix B). The form was collaboratively developed by the authors, based on previous studies evaluating the diagnostic and clinical influence of POCUS in internal medicine and hospitalist settings [64,72,75,7880], and adapted to the specific objectives of this study. However, the instrument was not formally validated, and no systematic psychometric evaluation was performed. In addition, because POCUS images were not recorded, independent verification of diagnostic accuracy, image quality, and related clinical decisions was not feasible.

Upon study completion, participants filled out a survey (Fig 1) assessing their satisfaction with POCUS training, its usefulness and relevance in clinical practice, confidence in performing POCUS, and any equipment-related challenges (S3 Appendix C).

Sample size

The sample size was calculated using the single-proportion formula for binary outcomes [81]. The primary outcome was defined as a change in the main diagnostic hypothesis and/or management plan—both coded as mutually exclusive categories (change vs. no change). Assumptions included a 95% confidence level, a 10 percentage-point margin of error, and an expected proportion of 20% based on prior studies [64,72,75,78,79], yielding a minimum of 62 forms (S2 Appendix B). Because multiple forms were submitted per physician, observations were treated as clustered. Accordingly, we applied a design-effect adjustment using an average cluster size of 5.5 forms per hospitalist (as observed in our data, presented below) and a conservative intraclass correlation coefficient of 0.10, resulting in an adjusted requirement of approximately 90 forms (S2 Appendix B).

Statistical analysis

Statistical analyses were performed using SigmaStat software (version 11.0) [82]. Likert scale responses, treated as ordinal categorical variables, were regrouped for simplicity: responses 1 and 2 were combined into value 1, response 3 was recoded as value 2, and responses 4 and 5 were grouped into value 3. The following variables were recoded according to this categorization approach: confidence in performing POCUS (S1 Appendix A); diagnostic confidence before and after POCUS and image quality (S2 Appendix B); as well as satisfaction, perceived usefulness, and importance of POCUS training; confidence in equipment use and image interpretation; and interest in future use of POCUS (S3 Appendix C).

Descriptive statistics were used to summarize the characteristics of the study participants and the collected data. Categorical variables were expressed as absolute and relative frequencies. To assess the distribution of continuous variables, the Shapiro-Wilk test was performed. Since none of the variables followed a normal distribution, continuous variables are presented as medians and the 25th and 75th percentiles. Spearman rank correlation was performed to explore associations between continuous and ordinal variables. A significance level of p < 0.05 was considered statistically significant for all analyses.

In addition, open-ended question responses and relevant closed-ended items from the POCUS clinical-use form (S2 Appendix B) were transcribed into a structured Excel sheet and coded by the principal investigator for each form. Open-ended items used in the analyses were: primary diagnostic hypothesis (pre/post-POCUS), differential diagnoses (pre/post-POCUS), planned management (pre/post-POCUS), expected findings, and POCUS findings. The closed-ended item used was the diagnostic confidence in the primary hypothesis (pre/post-POCUS). From these fields, we created binary indicators (yes/no) for: change in the primary diagnostic hypothesis; change in diagnostic confidence; addition of an alternative diagnosis; exclusion of an alternative diagnosis; incidental findings; and change in management. Change in diagnostic confidence was defined as a shift between the three recoded Likert categories (value 1 = Likert 1–2; value 2 = Likert 3; value 3 = Likert 4–5). These derived variables were summarized descriptively as categorical data, and no formal qualitative analysis was performed. Open-text responses were also reviewed to extract brief contextual information.

Results

Participants’ characteristics

Of the 26 hospitalists invited, 19 were enrolled. Participant demographics and characteristics are summarized in Table 1.

Table 1. Participants’ characteristics.

Characteristics Results (N = 19)
Median age – yr (IQR)a 29 (28.0; 31.0)
Gender
 Male – no. (%) 9 (47)
 Female – no. (%) 10 (53)
Nationality
 Brazilian – no. (%) 19 (100)
Undergraduate degree besides medicine 0
Specialization
 Internal Medicine only – no. (%) 15 (79)
 Additional specialization – no. (%) 4 (21)
Time passed since residency training – yr (IQR) a 3.0 (2.0; 3.75)
Formal training on POCUS
 Yes – no. (%) 5 (26)
POCUS usage prior to study – no. of times (IQR) a 15.0 (5.0; 30.0)
Self-confidence in POCUS – no. (%)
 Slightly or not at all confident 6 (31)
 Confident 7 (37)
 Very or extremely confident 6 (31)

The table presents demographic data and key characteristics, including age, gender distribution, nationality, academic background, and POCUS-related information.

aInterquartile ranges.

Spearman rank correlation analysis revealed a significant negative correlation was found between the time since residency completion and having formal POCUS training (R = −0.479; p = 0.0374). While a positive correlation trend was observed between the frequency of POCUS use prior to the study and participants’ self-confidence (R = 0.431; p = 0.0649), it did not reach statistical significance.

Satisfaction and perspectives of the application of POCUS

Fifty-three per cent (10/19) of participants completed the survey regarding training satisfaction and personal perspectives of the application of POCUS. All respondents were either “very “ or “extremely satisfied” with the training and agreed that it improved their ultrasound skills. Participants also highly valued POCUS, with all considering it “very” or “extremely useful” in clinical practice, and they all agreed that formal POCUS training is essential in medical residency programs. Additionally, everyone expressed strong interest in using POCUS in their future practice.

Regarding self-confidence while using POCUS on clinical scenarios, 60% (6/10) felt “confident,” 30% (3/10) felt “very” or “extremely confident,” and 10% (1/10) felt “slightly” or “not at all confident.” No correlation was observed between previous POCUS training and self-confidence levels (R = 0.150; p = 0.531). Participants reported challenges with ultrasound handling (4/10), image interpretation (5/10), need for more hands-on POCUS training for independent use (1/10), and limited time for using POCUS in clinical settings (3/10).

Clinical applications of POCUS

In total, 104 forms on POCUS clinical use were completed by 19 participants throughout the study, with a median of 2.0 forms [1.00; 5.75] per participant. Each form assessed one clinical encounter, which could demand more than one POCUS modality and clinical purposes. Among the 104 forms, POCUS was used by participants in various modalities and for multiple clinical purposes, as summarized in Figs 2 and 3, respectively.

Fig 2. POCUS modalities used.

Fig 2

The figure shows the distribution of POCUS use across various modalities, with lung being the most frequent (64 times), followed by heart (35 times) and inferior vena cava (25 times). More than one POCUS modality could be used in the same patient.

Fig 3. Clinical purposes.

Fig 3

The figure illustrates the number of times POCUS was employed for different diagnoses, with the most frequent uses for pulmonary congestion (47 times), urinary retention and pneumonia (18 times each) and pleural effusion (13 times).

The total time spent using POCUS was 935 minutes, with a median value of 10 minutes per patient [5.00; 10.00].

Regarding image quality, participants showed varying levels of satisfaction: 1/104 form(0.9%) was “slightly satisfied,” 21/104 forms (20%) were “satisfied,” and 82/104 forms (79%) were “very” or “extremely satisfied”.

Influence of POCUS on clinical reasoning and decision-making

Out of the 104 times POCUS were performed on patients, there was a change in the primary hypothesis in 38/104 scenarios (37%), while the primary diagnostic hypothesis remained unchanged in 66/104 scenarios (63%) (Fig 4). In 41/104 (39%) forms, there was a change in management, while in 63/104 (60%) management was maintained (Fig 5). In 48/104 forms (46%), there was an increase in diagnostic confidence, while in 56/104 (54%), confidence remained unchanged or decreased (Fig 4). Of the 104 completed forms, only 14/104 (13%) showed no influence of POCUS on clinical reasoning or decision-making. To assess this influence, we considered the following criteria: changes in the primary diagnostic hypothesis, changes in confidence level regarding the primary hypothesis, inclusion of a diagnostic hypothesis, exclusion of a hypothesis, identification of incidental findings, and modifications in clinical management.

Fig 4. Diagnostic influence.

Fig 4

The flowchart illustrates that in 37% of cases (38/104), the primary diagnostic hypothesis was modified following POCUS, whereas in 63% (66/104), it remained unchanged. Among cases with no change in hypothesis, diagnostic confidence increased in 36% (24/66), remained the same in 62% (41/66), and decreased in 2% (1/66). In cases where the hypothesis changed, 66% (25/38) showed a change in diagnostic confidence—96% of these (24/25) reported increased confidence, while 4% (1/25) reported decreased confidence. The remaining 34% (13/38) reported no change in confidence.

Fig 5. Clinical decision-making.

Fig 5

The flowchart illustrates that when the primary diagnostic hypothesis changed (38/104 cases), a change in clinical management occurred in 95% of those cases (36/38). When the hypothesis remained unchanged (66/104 cases), a change in management was observed in 8% of cases (5/66).

Among the 66 cases where the primary hypothesis was maintained, there was an increased level of confidence in 24/66 (36%). Twenty-two shifted from “confident” to “very confident” or “extremely confident,” and 2 shifted from “slightly confident” to “very confident.” No difference in the level of confidence was observed in 41/66 (62%). Amid cases with no confidence changes, 39/41 (95%) already had “high” or “extreme” confidence before performing POCUS, and the findings in these cases were consistent with expectations. The participants informed a decrease in their level of confidence in 1/66 (2%) scenario (Fig 4), shifting from “very confident” to “slightly confident”.

In 41/66 (62%) of the cases, participants used POCUS to rule out a diagnostic hypothesis, while 4/66 (6%) yielded incidental findings (Table 2). Only 1/66 (2%) case resulted in the addition of a diagnostic hypothesis based on POCUS findings (Table 2). Despite maintaining the primary diagnostic hypothesis, clinical management was altered in 5/66 (8%) cases (Fig 5). In 3 of these, POCUS was used to assess pulmonary congestion, and although findings were consistent with expectations, participants compared them with prior exams and determined that congestion was improving, opting not to intensify diuretic therapy. In 1 case, management was adjusted after ruling out an alternative hypothesis, and in another, an angiotomography was added, as POCUS was not considered sufficient to exclude pulmonary thromboembolism.

Table 2. POCUS influence on diagnostic reasoning and decision-making when the primary diagnostic hypothesis was maintained vs. changed.

Outcome Primary hypothesis maintained (N = 66) Primary hypothesis changed (N = 38)
Increased confidence 24 (36%) 24 (63%)
Ruling out hypothesis 41(62%) 32 (84%)
Incidental findings 4 (6%) 3 (8%)
Addition of hypothesis 1 (2%) 0 (0%)
Change in management 5 (8%) 36 (95%)

Distribution of diagnostic confidence, exclusion of diagnostic hypotheses, incidental findings, addition of diagnostic hypotheses, and management decisions, according to whether the primary hypothesis was maintained or changed.

Of the 38 forms where the primary diagnostic hypothesis changed, only 2/38 (5%) did not result in a change in management (Fig 5), as the initial planned strategy included both the pre-POCUS and post-POCUS primary diagnostic hypotheses. In 13/38 (34%) of these forms, the level of confidence in the primary diagnostic hypothesis remained unchanged (Fig 4). Among these, 2 participants were “confident,” 10 were “very confident” or “extremely confident” and 1 was “slightly confident” regarding the diagnosis. In the other 25/38 (66%) that altered their level of confidence in the primary diagnostic hypothesis, only 1/25 (4%) showed a decrease (Fig 4), shifting from “confident” to “slightly confident”. The remaining 24/25 (96%) recorded increases: 16 moved from “confident” to “very” or “extremely confident”, 5 from “slightly confident” to “very” or “extremely confident” “, 1 from “slightly confident” to “confident,” 2 from “not confident” to “very” or “extremely confident”.

In 3/38 forms (8%), incidental findings were recorded (Table 2); however, in only 1 case the finding was significant enough to add a new diagnostic hypothesis, which became the primary one, and resulted in changes in clinical management. In 32/38 forms (84%), a diagnostic hypothesis was ruled out (Table 2): 30 ruled out the primary diagnostic hypothesis, 1 ruled out an alternative diagnostic hypothesis, and 1 identified a more likely alternative diagnosis, replacing the primary hypothesis with this alternative diagnosis. In all cases where the primary diagnostic hypothesis changed, the POCUS findings differed from the participants’ initial expectations.

Discussion

Our study suggests that POCUS may influence clinical reasoning and decision-making. In 37% (38/104) cases, POCUS led to a modification of the primary diagnostic hypothesis, while in 39% (41/104), it resulted in changes to the clinical management plan. Additionally, an increase in diagnostic confidence was observed in 46% (48/104) of cases. Notably, in only 13% (14/104) of instances, POCUS had no discernible effect on clinical reasoning or decision-making.

These findings align with Lucas et al. [64], who reported management changes in 37% of the 210 participants who underwent cardiac ultrasound and conventional echocardiogram, but show a greater impact compared to Smallwood et al. [78], where only 34.8% of the 276 participants reported weekly decision-making changes and 10.3% daily. Compared to Cid-Serra et al. [72], who conducted a systematic review encompassing six studies with a total of 1,836 patients, our study found a higher rate of primary diagnostic hypothesis changes (37% vs. 18%), though clinical management influence was similar (39% vs. 37–52.1%). Similarly, our results exceeded those of Andersen et al. [79], who assessed 199 patients examined by medical residents. They reported changes in the primary diagnosis in 6.5% of cases. Our findings also indicate a greater diagnostic influence than Mjolstad et al. [75], who studied the diagnostic effect of cardiac and abdominal screening in 196 hospitalized patients and found a primary diagnosis change in 18.4% of cases.

In our study, even when the primary hypothesis remained unchanged (63% − 66/104), POCUS played an important role in strengthening diagnostic confidence, with certainty increasing in 36% (24/66) of these cases. The absence of changes in diagnostic confidence in some forms can be explained by the ceiling effect [8386]. Many participants who showed no variation in confidence were already classified as “very” or “extremely” confident in their initial diagnostic hypothesis before performing POCUS. In these cases, the ultrasound served primarily as a confirmatory tool, with little room for a significant increase in confidence. This phenomenon is expected in studies assessing confidence variation, as participants who start at a maximum level of certainty are less likely to demonstrate additional impact (ceiling effect), even when the tool used is effective.

POCUS also played an essential role in narrowing differential diagnoses, a particularly valuable function for hospitalists managing complex cases. When the primary hypothesis was maintained, POCUS helped rule out diagnoses in 62% (41/66) of cases, streamlining clinical decision-making. While management remained unchanged in most instances, POCUS still influenced care by guiding therapeutic choices in 8% (5/66), often through trend assessments or the exclusion of competing diagnoses. These findings highlight how POCUS not only supports diagnostic confirmation but also helps hospitalists refine differential diagnoses, potentially contributing to patient management decisions.

When the primary diagnostic hypothesis changed, POCUS had an even greater influence. In 95% (36/38) of these cases, management was modified, suggesting its direct influence on patient care. Additionally, in 84% (32/38) of cases, a diagnosis was ruled out, highlighting POCUS’s role in refining differential diagnoses—a key advantage for hospitalists navigating complex decision-making. Confidence levels also shifted notably, with 63% (24/38) of cases showing an increase in diagnostic certainty, further emphasizing POCUS’s role in strengthening clinical confidence.

Incidental findings were rare (6.7% – 7/104). Among these, only one case led to a complete change in the primary diagnosis, and another added an alternative diagnostic hypothesis. This rarity likely reflects that clinical reasoning is primarily guided by history-taking and physical examination, which help establish an initial diagnostic framework and set expectations for POCUS findings. Consequently, POCUS in our setting mainly served to confirm or refine initial hypotheses rather than introduce unexpected diagnoses. However, the identification of incidental findings also raises an important consideration. Such findings may divert the use of POCUS from its primary purpose—providing rapid answers to focused, binary clinical questions at the bedside—toward a more exploratory or comprehensive approach that it is not designed to fulfill. This highlights the need to maintain a clear diagnostic focus and to apply POCUS judiciously, ensuring it enhances rather than detracts from clinical reasoning. Notably, its role is particularly relevant in cases with intermediate pretest probability, where additional imaging can meaningfully influence decision-making by increasing certainty or ruling out alternative diagnoses.

Although limited data exist on the risk of incidentalomas in point-of-care ultrasound, there is growing concern in the literature about this issue [8789]. Unfocused or overly broad imaging may lead to incidental findings that trigger unnecessary investigations, patient anxiety, and resource use. However, when POCUS is guided by a specific clinical question and integrated into a structured diagnostic process, the chance of encountering incidentalomas is greatly reduced. This underscores the importance of training clinicians to use POCUS in a hypothesis-driven manner within the context of clinical reasoning. Moreover, healthcare teams and institutions should anticipate the possibility of incidental findings and develop protocols to manage them appropriately.

One of the key advantages of POCUS is its utility in settings with limited access to traditional radiologic imaging, such as the Transitional Care Unit at IPer. This unit has only plain radiography available, requiring inter-hospital transfer for specialized evaluations or additional imaging. This is also important in rural practices, humanitarian missions, and conflict zones [9093]. In these scenarios, POCUS might serve as an immediate diagnostic tool, enabling physicians to obtain crucial bedside information, optimizing clinical decision-making and management. Furthermore, given that the Transitional Care Unit at IPer primarily cares for elderly patients with multiple comorbidities and complex conditions, such as stroke, traumatic brain injury, and heart failure, the use of POCUS may help reduce reliance on additional imaging, minimize unnecessary transfers, and enhance overall care efficiency, ultimately facilitating a safe hospital discharge.

POCUS was applied across diverse clinical contexts, including cardiopulmonary, urinary, and hemodynamic assessments, underscoring its versatility in hospital-based practice. While our study did not evaluate the impact of a systematic approach, the breadth of its use observed is consistent with existing literature that supports structured protocols to improve the consistency and diagnostic accuracy of POCUS [9499]. Such approaches may enhance bedside clinical decision-making, particularly in acute care settings.

The participants were a homogeneous group in terms of academic background and clinical experience, consisting mostly of young professionals early in their careers. They had limited formal POCUS training, and their practical experience varied, leading to differences in confidence. These findings align with previous studies show that most Internal Medicine physicians use POCUS despite limited training [78,100].

Beyond the direct findings, our study highlights broader implications for hospital medicine. First, the frequent use of POCUS by hospitalists in diverse scenarios suggests that it is becoming integrated into routine reasoning processes. By influencing diagnostic hypotheses, management strategies, and confidence levels, POCUS appears to shape clinical reasoning in meaningful ways. However, most participants reported limited prior formal training, reflecting the well-documented gap in structured POCUS education within internal medicine residency programs [78,100]. This gap raises important concerns. The use of POCUS without adequate training may increase the risk of misinterpretation and diagnostic error, undermining the tool’s potential benefits. Previous studies have emphasized that appropriate supervision, competency standards, and validated curricula are essential to ensure safe and effective integration of POCUS into practice [78,100103]. Therefore, our findings reinforce the need for internal medicine societies and residency program directors to systematically consider how POCUS training should be incorporated into education and clinical governance frameworks.

Furthermore, the survey results revealed a highly positive perception of brief POCUS orientation session. All participants expressed satisfaction, emphasizing its value in clinical practice and unanimously supporting its inclusion in residency programs and continuing medical education programs. However, barriers such as ultrasound handling, image interpretation, limited supervision, and clinical time constraints were noted.

Although no significant correlations were found between prior formal training and self-confidence, it is important to highlight that self-confidence in using POCUS in clinical scenarios was assessed in only 10 out of 19 respondents (53%), which limits the strength of conclusions regarding confidence and should be considered a limitation.

This study has some limitations. First, we did not assess the impact of POCUS on robust clinical outcomes such as length of stay, readmission rates, or mortality. Second, the small sample size may limit the generalizability of the results and increase susceptibility to bias. Third, POCUS images were not recorded, precluding independent review of acquisition and interpretation, and preventing evaluation of diagnostic accuracy, interobserver reliability, or concordance with gold-standard criteria. Decisions were therefore based solely on participants’ self-reported findings, which may have led to overestimation of perceived safety and influence. In addition, process variables such as exam duration and image quality were also self-reported rather than objectively measured. Exam time was estimated by participants, raising the risk of recall bias, while image quality was judged subjectively without external validation, introducing self-report bias. Another limitation relates to the data collection form, which was developed by the authors based on previous studies and study objectives but was not formally validated; as such, the accuracy and interpretability of the captured constructs may be constrained. Finally, open-ended responses were categorized by a single investigator without independent coding or inter-rater reliability assessment, which may introduce subjective bias. These aspects should be considered when interpreting the feasibility and quality of POCUS implementation in our study.

Despite these limitations, our findings underscore the meaningful influence of POCUS on the clinical practice of hospitalists, highlighting its important role in refining clinical reasoning and decision-making. The findings indicate that POCUS contributed to a change in the primary diagnostic hypothesis in 37% (38/104) of cases, a modification in clinical management in 39% (41/104), and an increase in diagnostic confidence in 46% (48/104) of cases. Additionally, the use of POCUS in ruling out differential diagnoses may contribute to refining clinical reasoning, which is often complex in hospital medicine. Moreover, POCUS has potential to assist hospitalists in settings with limited access to imaging exams, where bedside ultrasound serves as a useful adjunct to support timely decision-making, potentially guiding patient care and management.

Conclusion

This study suggests that POCUS may influence diagnostic hypotheses, clinical management decisions, and physician confidence. It appeared to contribute to clinical reasoning and decision-making in many cases, while in some instances it showed little or no effect.

These findings add to the limited body of literature on the application of POCUS in internal medicine, indicating its potential value as a bedside modality. By illustrating its possible influence on clinical judgment and reasoning—particularly in resource-limited settings—this study provides preliminary evidence that POCUS may serve as a useful adjunct in hospitalist practice. Future research should employ stronger designs, such as randomized controlled trials or controlled before-and-after studies with validated outcome measures and objective performance data, to determine whether enhancements in clinical reasoning facilitated by POCUS translate into measurable improvements in patient outcomes and healthcare efficiency, such as reduced hospital length of stay, readmission rates, or mortality.

Overall, the results should be interpreted as exploratory, emphasizing feasibility and potential utility rather than efficacy. As clinical demands continue to evolve, incorporating effective bedside approaches like POCUS could ultimately play a supportive role in improving patient care.

Supporting information

S1 Appendix. A. Form on participants’ personal data.

Questionnaire completed by participants before the study. It collects demographic data, academic background, previous experience with point-of-care ultrasound (POCUS), and self-assessed confidence in performing and interpreting POCUS examinations.

(DOCX)

pone.0338202.s001.docx (26.1KB, docx)
S2 Appendix. B. Form on POCUS clinical use.

Structured form used by participants after each POCUS application during clinical practice. It documents the clinical question, diagnostic hypothesis, confidence levels before and after POCUS, selected ultrasound modalities, image quality, findings, and subsequent changes in diagnosis or management.

(DOCX)

pone.0338202.s002.docx (32.8KB, docx)
S3 Appendix. C. Form on participants’ impressions and satisfaction survey.

Survey assessing participants’ satisfaction with the POCUS course, perceived usefulness in clinical practice, importance of formal training, confidence levels after training, future interest in using POCUS, and perceived barriers to its implementation in the clinical setting.

(DOCX)

pone.0338202.s003.docx (55.2KB, docx)

Acknowledgments

We would like to thank Instituto Perdizes for their invaluable support in conducting this research.

Data Availability

All relevant data are within the paper and its Supporting Information files.

Funding Statement

We would also like to acknowledge that this study was financially supported by the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) through the Thematic Project (Grant nº 2018/02537-05). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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Decision Letter 0

Biswabandhu Jana

3 Jun 2025

Dear Dr. Dias Souza,

Thank you for submitting your manuscript to PLOS ONE. After careful consideration, we feel that it has merit but does not fully meet PLOS ONE’s publication criteria as it currently stands. Therefore, we invite you to submit a revised version of the manuscript that addresses the points raised during the review process.

Please submit your revised manuscript by Jul 18 2025 11:59PM. If you will need more time than this to complete your revisions, please reply to this message or contact the journal office at plosone@plos.org . When you're ready to submit your revision, log on to https://www.editorialmanager.com/pone/ and select the 'Submissions Needing Revision' folder to locate your manuscript file.

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We look forward to receiving your revised manuscript.

Kind regards,

Biswabandhu Jana, Phd

Academic Editor

PLOS ONE

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Additional Editor Comments:

The paper presents a point-of-care ultrasound (POCUS) on diagnostic purposes. The study needs a major revision.

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Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. Is the manuscript technically sound, and do the data support the conclusions?

Reviewer #1: Partly

Reviewer #2: Yes

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2. Has the statistical analysis been performed appropriately and rigorously? -->?>

Reviewer #1: Yes

Reviewer #2: Yes

**********

3. Have the authors made all data underlying the findings in their manuscript fully available??>

The PLOS Data policy

Reviewer #1: Yes

Reviewer #2: Yes

**********

4. Is the manuscript presented in an intelligible fashion and written in standard English??>

Reviewer #1: Yes

Reviewer #2: Yes

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Reviewer #1: The authors present a prospective study investigating the impact of Point-of-Care Ultrasonography (POCUS) on the clinical reasoning, management strategies, and confidence levels of hospitalists in a transitional care unit.

This research is highly relevant given the increasing integration of POCUS into clinical practice, not-limited to settings with limited access to advanced imaging. The study's finding that POCUS significantly influences clinical reasoning and decision-making among hospitalists is a valuable contribution to the literature.

Specific Feedback and Suggestions for Improvement

- Study Design: While described as an "exploratory prospective study," a more precise classification such as a "descriptive prospective study" would accurately reflect the study's aim to observe and describe the influence of POCUS.

- Sample Size Calculation: Clarification is needed regarding the odds ratio of 4 used in the sample size calculation. Please specify which "form" is being discussed and provide a detailed explanation of the rationale behind all values and assumptions considered for known or unknown variables in the calculation.

- Statistical Analysis: It would be beneficial to explicitly state for which specific variables the Likert scale responses were used and subsequently grouped for analysis.

- Impact of Brief Training: The study's premise that a single two-hour POCUS training session can instill confidence for accurate diagnosis, especially without prior formal exposure, warrants further discussion. The results indicate that 26% of participants had previous ultrasound training (median of 15 POCUS examinations prior to the study), with 68% already reporting confidence in using POCUS. This suggests a potential confounding effect from these pre-trained individuals. It would be valuable to analyze the impact of this short training separately for individuals with no prior ultrasound experience to better isolate its true effect.

- Learning Curve and Supervision: The authors' own finding that "No significant difference was found between pre-test and post-test performance (T = 105.00; p = 0.970), indicating that our single short-duration POCUS training did not improve participants' performance in image interpretation" reinforces the understanding that POCUS, despite its short learning period, typically requires supervision and multiple hands-on exposures for proficiency in image interpretation. We did not find any mention of how the POCUS findings were verified.

- Pre- and Post-Training Test Participation: The limited participation rates for the pre- and post-training objective structured video examinations (OSVE) (63% and 53% respectively) could potentially dilute the observed impact of the training on participants' knowledge. This limitation should be acknowledged when discussing the effectiveness of the training.

- Verification of Diagnostic and Management Changes: The methodology for verifying the accuracy of diagnosis and management decision changes needs to be clearly explained. How were these changes objectively assessed and confirmed?

- Timing of Feedback: The timing of feedback regarding the usefulness of POCUS in clinical practice is crucial. If the feedback was collected immediately post-training, it may not accurately reflect the sustained utility or impact. Please clarify when this feedback was gathered.

- Self-Confidence Evaluation: It appears that self-confidence while using POCUS in clinical scenarios was evaluated in only 10 out of 19 respondents (50% of the study subjects). This smaller sample size for confidence evaluation should be highlighted as a limitation.

Results Presentation

- Redundancy in Reporting: Avoid repeating numerical values in the text if they are already clearly presented in tables or figures (e.g., Figure 2, 3, 4, Table 2).

- POCUS Utilization Data: While the presentation of organ evaluations (lines 237-246) provides some detail, a more impactful representation of POCUS clinical uses could be achieved by focusing on the 167 items for clinical uses depicted in lines 251-258, as these directly address the clinical questions POCUS is designed to answer.

- Time Efficiency Analysis: In the "Time spent per number of modalities" assessment, the results section only states "The total time spent using POCUS was 935 minutes, with a mean value of 9 minutes per patient and 5.3 minutes per modality performed." It would be valuable to evaluate the effect of prior POCUS exposure on time efficiency.

- Elaboration on Diagnostic Hypothesis Changes: In Results Line 330, the statement "In all cases where the primary diagnostic hypothesis changed, the POCUS findings were inconsistent with what was expected" requires further explanation for clarity.

- Table 2: Consider whether Table 2 is truly necessary, as the data it presents does not appear to reflect significant findings that warrant a separate table.

- Figures and Tables Placement: For ease of review, tables and figures should be provided separately from the main text, rather than embedded within it.

Discussion and Conclusion

- Systematic use of POCUS: The discussion could benefit from emphasizing the importance of systematic POCUS use to enable standardized application of this widely available tool. Referencing relevant literature on systematic POCUS approaches in various subspecialties (e.g., Pokharel B. Systematic use of Point of Care Ultrasound in Neurosurgical Intensive Care Unit: a practical approach. Quant Imaging Med Surg 2023;13(4):2287-2298. https://dx.doi.org/10.21037/qims-22-667) could strengthen this point.

- Incidental Diagnoses: The finding of incidental diagnoses in 8% of cases is interesting, but it also raises a valid concern. POCUS is primarily designed to answer specific clinical questions in a binary format, not for comprehensive organ or system evaluations. This finding could be discussed in the context of the potential for POCUS use to become distracted from its primary purpose.

- Learning Curve for Less Experienced Individuals: In the Discussion, line 474, the deduction that "participants showed a trend toward increased efficiency in POCUS execution over time, particularly those with less prior exposure, suggesting a learning curve" is an important point. However, to fully support this claim, the authors should separate the analysis of efficiency for groups with and without prior POCUS experience.

- Conclusion: The conclusion should primarily summarize the study's key findings based on the results, and limitations should not be reiterated in this section.

Reviewer #2: The article titled " Assessing the Clinical Relevance of Point-of-care Ultrasound for Hospitalists: Impact on Clinical Reasoning and Decision-making" presents an insightful exploration of how Point-of-Care Ultrasound (POCUS) influences the clinical decision-making process among hospitalists. The study is well-structured and provides valuable data on the integration of POCUS in clinical practice.

This study is an exploratory prospective analysis carried out at the Transitional Care Unit of Instituto Perdizes, which is part of a larger hospital complex in the city of São Paulo, São Paulo, Brazil. This setting is noteworthy as it highlights the challenges faced in resource-limited environments, where access to advanced imaging options is more limited (only X-ray) compared to other institutions.

Participants consisted of 19 Brazilian hospitalists with undergraduate degrees in medicine only, varying levels of prior POCUS experience, and an average of 15 prior POCUS uses. Regarding self-confidence, 68% of the hospitalists reported being confident, or very confident, in using POCUS. This experience enables a comprehensive evaluation of how Point-of-Care Ultrasound (POCUS) impacts clinical reasoning.

The findings suggest that the use of POCUS by hospitalists changed the primary diagnostic hypothesis in 37% of cases, and the management altered in 39% of encounters. While the primary diagnosis remained consistent in 63% of cases, the use of POCUS increased diagnostic confidence in 36% of these instances and helped rule out alternative diagnoses in 62% of cases. In only 13% of cases, the use of POCUS had no noticeable impact on clinical reasoning or decision-making. Although a brief training session did not significantly improve Objective Structured Video Exam (OSVE) scores, participants expressed high satisfaction with the training and acknowledged the utility of POCUS.

The findings highlight the potential of POCUS to enhance clinical reasoning and decision-making, especially in situations where access to advanced imaging is restricted. In this context, the use of POCUS is essential for improving patient care and outcomes in transitional care units.

Although the study did not evaluate the effects of POCUS on length of stay, readmission rates, and mortality, it is important to note that these outcomes may vary depending on patient populations and clinical contexts.

The study's methodology, which includes detailed documentation of POCUS use and its impact on clinical decisions, provides a good framework for future research in this field.

In conclusion, this article greatly improves our understanding of the role of Point-of-Care Ultrasound (POCUS) in clinical settings with limited resources. It successfully combines empirical data with practical insights, making it a valuable resource for hospitalists and healthcare professionals who aim to enhance their diagnostic processes. Future research could expand on these findings by exploring the long-term outcomes related to the use of POCUS in different clinical situations.

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Reviewer #1: Yes:  Prof Dr Amit Thapa

Reviewer #2: No

**********

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PLoS One. 2025 Dec 18;20(12):e0338202. doi: 10.1371/journal.pone.0338202.r002

Author response to Decision Letter 1


1 Jul 2025

Response to Reviewer #1

We would like to sincerely thank Reviewer #1 for their thoughtful and constructive comments, which have greatly improved the clarity, rigor, and overall quality of our manuscript. We carefully considered all the suggestions and revised the text accordingly. Below, we provide detailed responses to each comment.

Study Design

Comment: While described as an "exploratory prospective study," a more precise classification such as a "descriptive prospective study" would accurately reflect the study's aim to observe and describe the influence of POCUS.

Response: We thank the reviewer for this suggestion. We fully agree that "descriptive prospective study" more accurately reflects the design and objectives of our work. Accordingly, we revised the Methods section (line 110), replacing the previous phrasing with: “This is a descriptive prospective study”. We also made the same adjustment in the Abstract (line 33) to ensure consistency throughout the manuscript.

Sample Size Calculation

Comment: Clarification is needed regarding the odds ratio of 4 used in the sample size calculation. Please specify which "form" is being discussed and provide a detailed explanation of the rationale behind all values and assumptions considered for known or unknown variables in the calculation.

Response: We sincerely thank the reviewer for the careful attention dedicated to our sample size calculation, and for this valuable observation. The text we provided in the first manuscript was inaccurate. As we first designed our research project and contacted a statistician, they suggested an approach based on the McNemar test. After further discussion among the investigators, we decided to pursuit a different approach. Unfortunately, the first version of the manuscript sent to your consideration mistakenly presented the original statistical approach.

The investigators defined along with a consultant statistician a more suitable sample size approach: the formula for estimating proportions in binary nominal outcomes, with finite population correction. This method better reflects our primary outcome, defined a priori as the occurrence of a change in the main diagnostic hypothesis and/or management plan.

The updated file of our manuscript presents the rationale and the assumptions in the Methods section, between lines 165 and 174.

Statistical Analysis

Comment: It would be beneficial to explicitly state for which specific variables the Likert scale responses were used and subsequently grouped for analysis.

Response: We thank the reviewer for this suggestion. We agree that these variables were not clearly expressed in this section of the manuscript, and with the importance of its specification. We revised the Methods section to clearly indicate which variables were measured using Likert scales and how these responses were grouped for analysis. The following sentence was added (lines 179–184): The following variables were recoded according to this categorization approach: confidence in performing POCUS (S1 appendix A); diagnostic confidence before and after POCUS and image quality (S2 appendix B); as well as satisfaction, perceived usefulness, and importance of POCUS training; confidence in equipment use and image interpretation; and interest in future use of POCUS (S3 appendix C).

Impact of Brief Training

Comment: The study's premise that a single two-hour POCUS training session can instill confidence for accurate diagnosis, especially without prior formal exposure, warrants further discussion. The results indicate that 26% of participants had previous ultrasound training (median of 15 POCUS examinations prior to the study), with 68% already reporting confidence in using POCUS. This suggests a potential confounding effect from these pre-trained individuals. It would be valuable to analyze the impact of this short training separately for individuals with no prior ultrasound experience to better isolate its true effect.

Response: We thank the reviewer for this insightful observation. Indeed, we agree this hypothesis is reasonable and warrants further statistical analysis. This prompted us to further explore the potential confounding effect of prior POCUS experience. Following this suggestion, we conducted an additional analysis comparing OSVE performance between participants with and without prior formal POCUS training.

The “Statistical analysis” section was updated to include this new step (lines 196-197): “Independent samples t-tests were conducted to compare participants' pre-test and post-test OSVE performance according to prior formal POCUS training.”

The results of this analysis were incorporated into the revised manuscript (lines 225–234): “To assess the relationship between participants’ performance on the pre- and post-test OSVE and prior POCUS training, participants were divided into two groups: those with prior POCUS training and those without. No difference was observed when comparing pre-test scores (T = 0.643; p = 0.535), post-test scores (T = -0.0570; p = 0.956), and the change between pre and post-test scores (T = 0.522; p = 0.616) among both groups. To evaluate whether prior hands-on POCUS experience—measured by the number of times participants reported having performed POCUS before the study—was associated with the change in OSVE scores, we applied the Spearman rank-order correlation. No significant relationship was found between the number of prior POCUS procedures and score variation (R < 0.001; p = 1.000).”

These findings were also addressed in the Discussion (lines 476–485): “This study found no statistically significant differences in OSVE performance between participants with and without prior formal POCUS training, either in the pre-test, post-test, or in the variation between them. Likewise, no correlation was observed between the number of prior POCUS procedures performed and changes in test scores. These findings suggest that previous exposure to POCUS—whether formal or practical—was not associated with better performance in this structured assessment. A possible explanation is that the OSVE may evaluate broader skills such as clinical reasoning and integration of ultrasound findings into decision-making, which may not be fully developed through prior training alone. Additionally, variability in the type and depth of previous training, as well as the small sample size, may have limited the detection of differences.”

Learning Curve and Supervision

Comment: The authors' own finding that "No significant difference was found between pre-test and post-test performance (T = 105.00; p = 0.970), indicating that our single short-duration POCUS training did not improve participants' performance in image interpretation" reinforces the understanding that POCUS, despite its short learning period, typically requires supervision and multiple hands-on exposures for proficiency in image interpretation. We did not find any mention of how the POCUS findings were verified.

Response: We thank the reviewer for this important observation. We realize that our original wording may have caused ambiguity regarding what was meant by "participants' performance in image interpretation." The phrase refers specifically to participants’ performance in interpreting ultrasound images presented in the Objective Structured Video Exam (OSVE), not the interpretation of POCUS images they acquired during the study. To clarify this, we revised the sentence in the Results section (lines 463–465) as follows: “No significant difference was found between pre-test and post-test performance (T = 105.00; p = 0.970), indicating that our single short-duration POCUS training did not improve participants' performance in image interpretation on the OSVE.”

We appreciate the reviewer’s highlighting of these points, which helped improve clarity and transparency in our manuscript.

Pre- and Post-Training Test Participation

Comment: The limited participation rates for the pre- and post-training objective structured video examinations (OSVE) (63% and 53% respectively) could potentially dilute the observed impact of the training on participants' knowledge. This limitation should be acknowledged when discussing the effectiveness of the training.

Response: We thank the reviewer for this observation. We agree that the limited participation rates in the pre- and post-training OSVE assessments may have influenced the measured impact of the training intervention. To address this, we have explicitly acknowledged this limitation in the Discussion section (lines 471–473), adding the following statement: “Additionally, the limited participation rates in the pre- and post-training OSVE—63% and 53% respectively—may have diluted the observed impact of the training on participants' knowledge.”

This acknowledgment emphasizes the potential influence of incomplete data on the evaluation of the training’s effectiveness.

Verification of Diagnostic and Management Changes

Comment: The methodology for verifying the accuracy of diagnosis and management decision changes needs to be clearly explained. How were these changes objectively assessed and confirmed?

Response: We thank the reviewer for highlighting this important point. We agree that the manuscript did not clearly specify the verification process for the accuracy of diagnostic findings and management decisions influenced by POCUS. To address this, we have added a clarifying statement in the Methods section (lines 151–153), as follows: “Since POCUS images were not recorded, it was not possible to independently verify the accuracy of the diagnostic findings or the clinical management decisions informed by them.”

This addition acknowledges the study limitation regarding objective verification and improves transparency about the methodology used.

Timing of Feedback

Comment: The timing of feedback regarding the usefulness of POCUS in clinical practice is crucial. If the feedback was collected immediately post-training, it may not accurately reflect the sustained utility or impact. Please clarify when this feedback was gathered.

Response: We thank the reviewer for pointing out this important issue. Upon review, we recognized that the original manuscript did not clearly specify the timing of the participants’ impressions and satisfaction survey. To clarify, we removed the paragraph stating that the survey was completed immediately post-training. We replaced it with a more accurate description in lines 160–163: “Upon study completion, participants completed a survey (Fig 1) assessing their satisfaction with POCUS training, its usefulness and relevance in clinical practice, confidence in using the method, and any challenges encountered with the equipment (S3 appendix C).”

This revision reflects that feedback was collected at the end of the study, thus capturing a more comprehensive perspective on the sustained utility and impact of POCUS in clinical practice. Accordingly, we updated Figure 1 to represent this change. Additionally, due to the change in appendix citations, we reversed the order of appendices B and C to maintain consistency in the text.

We believe these clarifications enhance transparency and address the reviewer’s concern about the timing of feedback collection.

Self-Confidence Evaluation

Comment: It appears that self-confidence while using POCUS in clinical scenarios was evaluated in only 10 out of 19 respondents (50% of the study subjects). This smaller sample size for confidence evaluation should be highlighted as a limitation.

Response: We appreciate the reviewer’s comment regarding the sample size for the self-confidence evaluation. We agree that assessing self-confidence in only 10 out of 19 participants (53%) represents a limitation that restricts the generalizability and strength of any conclusions drawn from this analysis. Accordingly, we have explicitly acknowledged this limitation in the Discussion section (lines 496–499):

“Although no significant correlations were found between prior formal training and self-confidence, it is important to highlight that self-confidence in using POCUS in clinical scenarios was assessed in only 10 out of 19 respondents (53%), which limits the strength of conclusions regarding confidence and should be considered a limitation.”

This addition ensures transparency regarding the interpretation of our findings related to participants’ self-confidence using POCUS in clinical practice.

Results Presentation

Redundancy in Reporting

Comment: Avoid repeating numerical values in the text if they are already clearly presented in tables or figures (e.g., Figure 2, 3, 4, Table 2).

Response: We thank the reviewer for this important suggestion regarding the clarity and conciseness of our results presentation. In response, we have revised the manuscript to reduce redundancy between the text and the tables/figures, as follows:

• For Table 1, we removed detailed numeric descriptions from the text and instead provided a concise summary statement (lines 202–203):

“Participant demographics and characteristics are summarized in Table 1.”

• Similarly, for Table 2, we streamlined the description (lines 339–341):

“To assess participants’ performance over the course of the study, we compared the first and last POCUS clinical impact forms completed by each participant. Final forms were completed by 73.7% of them. Summary data are presented in Table 2.”

• For Figures 2 and 3, we opted to avoid repeating detailed data in the text and included a general reference to these figures (lines 254–255):

“Among the 104 forms, POCUS was used by participants in various modalities and for multiple clinical purposes, as summarized in Figs 2 and 3, respectively.”

• However, for Figures 1, 4, and 5, we chose to maintain both textual descriptions and the figures themselves. We believe this approach enhances the reader’s understanding of the study methods and results, as the textual explanations complement the visual data presentation.

These revisions improve the manuscript’s readability by eliminating unnecessary repetition while preserving clarity and comprehension.

POCUS Utilization Data

Comment: While the presentation of organ evaluations (lines 237–246) provides some detail, a more impactful representation of POCUS clinical uses could be achieved by focusing on the 167 items for clinical uses depicted in lines 251–258, as these directly address the clinical questions POCUS is designed to answer.

Response: We appreciate the reviewer’s insightful suggestion to emphasize the clinical uses of POCUS over organ-specific evaluations, as this better reflects the practical utility of the method in answering bedside clinical questions. In accordance with this recommendation, we have revised the discussion section by removing the paragraph originally between lines 501 and 506, which described the frequency of organ evaluations. This paragraph was replaced by a more concise statement (lines 451–453):

“POCUS was applied across diverse clinical contexts, including cardiopulmonary, urinary, and hemodynamic assessments, underscoring its versatility in hospital-based practice.”

This change allows us to focus the discussion on the broad clinical applicability of POCUS, highlighting its role in guiding patient care decisions rather than solely detailing the anatomical areas assessed. We believe this adjustment better captures the essence of POCUS utilization and aligns with the reviewer’s recommendation.

Time Efficiency Analysis

Comment: In the "Time spent per number of modalities" assessment, the results section only states "The total time spent using POCUS was 935 minutes, with a mean value of 9 minutes per patient and 5.3 minutes per modality performed." It would be valuable to evaluate the effect of prior POCUS exposure on time efficiency.

Response: We appreciate the reviewer’s suggestion to further explore the influence of prior POCUS exposure on time efficiency. Accordingly, we conducted an additional analysis, which has been incorporated into the Results section (lines 264–273). Specifically, we assessed the total time spent per modality in relation to participants’ previous POCUS experience, measured both by self-reported number of exams performed before the study and formal training status.

Our findings in

Attachment

Submitted filename: Response to Reviewers.docx

pone.0338202.s004.docx (32.7KB, docx)

Decision Letter 1

Biswabandhu Jana

25 Aug 2025

Dear Dr. Dias Souza,

Thank you for submitting your manuscript to PLOS ONE. After careful consideration, we feel that it has merit but does not fully meet PLOS ONE’s publication criteria as it currently stands. Therefore, we invite you to submit a revised version of the manuscript that addresses the points raised during the review process.

Please submit your revised manuscript by Oct 08 2025 11:59PM. If you will need more time than this to complete your revisions, please reply to this message or contact the journal office at plosone@plos.org . When you're ready to submit your revision, log on to https://www.editorialmanager.com/pone/ and select the 'Submissions Needing Revision' folder to locate your manuscript file.

  • A rebuttal letter that responds to each point raised by the academic editor and reviewer(s). You should upload this letter as a separate file labeled 'Response to Reviewers'.

  • A marked-up copy of your manuscript that highlights changes made to the original version. You should upload this as a separate file labeled 'Revised Manuscript with Track Changes'.

  • An unmarked version of your revised paper without tracked changes. You should upload this as a separate file labeled 'Manuscript'.

If you would like to make changes to your financial disclosure, please include your updated statement in your cover letter. Guidelines for resubmitting your figure files are available below the reviewer comments at the end of this letter.

If applicable, we recommend that you deposit your laboratory protocols in protocols.io to enhance the reproducibility of your results. Protocols.io assigns your protocol its own identifier (DOI) so that it can be cited independently in the future. For instructions see: https://journals.plos.org/plosone/s/submission-guidelines#loc-laboratory-protocols . Additionally, PLOS ONE offers an option for publishing peer-reviewed Lab Protocol articles, which describe protocols hosted on protocols.io. Read more information on sharing protocols at https://plos.org/protocols?utm_medium=editorial-email&utm_source=authorletters&utm_campaign=protocols .

We look forward to receiving your revised manuscript.

Kind regards,

Biswabandhu Jana, Phd

Academic Editor

PLOS ONE

Journal Requirements:

If the reviewer comments include a recommendation to cite specific previously published works, please review and evaluate these publications to determine whether they are relevant and should be cited. There is no requirement to cite these works unless the editor has indicated otherwise. 

[Note: HTML markup is below. Please do not edit.]

Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

Reviewer #3: (No Response)

Reviewer #4: All comments have been addressed

**********

2. Is the manuscript technically sound, and do the data support the conclusions??>

Reviewer #3: No

Reviewer #4: No

**********

3. Has the statistical analysis been performed appropriately and rigorously? -->?>

Reviewer #3: Yes

Reviewer #4: No

**********

4. Have the authors made all data underlying the findings in their manuscript fully available??>

The PLOS Data policy

Reviewer #3: Yes

Reviewer #4: Yes

**********

5. Is the manuscript presented in an intelligible fashion and written in standard English??>

Reviewer #3: Yes

Reviewer #4: Yes

**********

Reviewer #3: Congratulations to the authors of the manuscript titled 'Assessing the clinical relevance of point of care ultrasound for hospitalists: impact on clinical reasoning and decision making'. The authors have done an excellent job of responding to the comments from the previous reviewers. I must specify that I was not part of the previous set of review. The authors have done a good job of presenting the data and discussing it. The language is clear, and illustrative of the work that has been done. The work presentation itself is generally high quality and i want to congratulate the authors for this.

However, I do have significant concerns with the study design and the conclusions that are drawn from them. Please see my issues here:

1. POCUS education for 120 minutes without a follow up period of verification of competency or progression of skill.

2. After completion of the education, the participant images were not reviewed for accuracy, completeness or appropriate interpretation in the clinical context.

Due to these two issues, the current conclusion is that 120 mins of education without verification of skills is appropriate for patient management and skill application into patient care. This is extremely concerning for me because there is a likelihood of harm reaching to the patient. I am not comfortable with that conclusion and possible outcome.

Due to these limitations, I personally cannot suggest that the manuscript is accepted for publication.

Reviewer #4: (No Response)

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Reviewer #3: No

Reviewer #4: No

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Attachment

Submitted filename: PlosOne_Report.docx

pone.0338202.s005.docx (15.7KB, docx)
PLoS One. 2025 Dec 18;20(12):e0338202. doi: 10.1371/journal.pone.0338202.r004

Author response to Decision Letter 2


6 Oct 2025

Response to Reviewer #3

We thank the reviewer for the insightful comments and for highlighting essential patient-safety considerations related to POCUS training and verification. We revised the manuscript to clarify the scope of our educational intervention and the interpretability of our findings.

Comment 1:

“POCUS education for 120 minutes without a follow up period of verification of competency or progression of skill.”

We agree that a brief session does not establish technical competency. In the revised Methods, we clarify that the 120-minute session functioned as a brief orientation to align participants on basic Point-of-care Ultrassound (POCUS) concepts, terminology, and device operation, and was not designed to establish or verify technical competency. No follow-up skill verification or assessment of progression was performed, and the session was not analyzed as an intervention. Additionally, to streamline the manuscript and keep focus on the primary objectives, we removed the Objective Structured Video Examination (OSVE) and all related pre/post analyses, as well as the exploratory comparison between the first and last POCUS forms and the derived efficiency metric (“time spent per number of modalities”). These changes prevent secondary analyses of educational (OSVE) and process measures from overshadowing our primary per-encounter descriptions of how clinician-directed POCUS related to diagnostic hypotheses, management decisions, and confidence during routine care.

Comment 2:

“After completion of the education, the participant images were not reviewed for accuracy, completeness or appropriate interpretation in the clinical context.”

We agree and have made this limitation explicit in both the Methods and the Limitations sections, and we also reiterate it in the Conclusion to ensure a cautious interpretation of the findings.

Comment 3:

“Due to these two issues, the current conclusion is that 120 mins of education without verification of skills is appropriate for patient management and skill application into patient care. This is extremely concerning for me because there is a likelihood of harm reaching to the patient. I am not comfortable with that conclusion and possible outcome.”

We fully agree and have revised the entire manuscript to adopt a more cautious framing. The revised text emphasizes feasibility and perceived influence on clinical reasoning, rather than suggesting effectiveness of training. We now state that stronger study designs with validated protocols and competency assessment are required to confirm these findings.

Response to Reviewer #4

Comment 1:

“The chosen study design, however, requires clarification and more precise description. The manuscript currently refers to the study in broad terms, but given the structure — participants undergoing baseline assessment, receiving an educational intervention (POCUS training), and then being reassessed with both OSVE and questionnaires — this aligns most accurately with a prospective quasi-experimental before-and-after design. This classification is important because the study does involve an intervention and measures outcomes before and after its implementation, but it does not include randomization or a parallel control group. Calling it a ‘descriptive prospective study’ would be misleading, as descriptive studies do not assess causal effects or intervention impact. Properly labeling the design as quasi-experimental strengthens methodological transparency and allows readers to appropriately interpret the results.”

We thank the reviewer for the careful assessment. In response, we removed all pre/post analyses related to the educational session, including any Objective Structured Video Examinations (OSVE) based comparisons, to keep the study focused on our primary objective. The educational session is now presented exclusively as introductory orientation (not an analyzed intervention). Importantly, the OSVE is described only as exposure to standardized clinical vignettes containing Point-of-care Ultrassound (POCUS) images to align participants’ familiarity with image-based content; no OSVE scores or pre/post contrasts enter our analyses. With these changes, the manuscript no longer evaluates intervention effects and does not estimate causal impact. The study therefore fits a prospective descriptive observational design. We also added clarifying language to prevent any implication of a quasi-experimental before-and-after framework.

Comment 2:

“I have concerns regarding the measurement of two key process variables: (a) time spent performing the POCUS exam, and (b) quality of images obtained. According to the data collection form, both variables were captured through participant self-report rather than objective measurement. For time spent, it is unclear whether this was based on a stopwatch or structured observation; as presented, the data seem to rely entirely on participants’ subjective estimation. Similarly, the assessment of image quality was based on participants’ own descriptions without saving or independently reviewing the images. This introduces a high risk of recall bias and self-report bias. Moreover, not storing images for subsequent blinded quality review represents a missed opportunity to validate this outcome objectively. These limitations should be explicitly acknowledged in the manuscript, as they constrain the strength of the conclusions regarding feasibility and quality of POCUS implementation.”

We thank the reviewer for these important observations. In the revised manuscript, we clarified in the Methods that both process variables were self-reported rather than objectively measured. Specifically, we now state that exam duration was reported as an estimated time by the participant, not measured with a stopwatch or structured observation. Similarly, we emphasize that image quality was based solely on participants’ self-reported perceptions. In addition, we made it explicit that POCUS images were not recorded, which precluded independent verification of acquisition quality, diagnostic accuracy, or related management decisions.

We also revised the Discussion to explicitly acknowledge these issues as limitations. We highlight that reliance on participants’ estimations introduces a risk of recall bias, while self-reported image quality without external review introduces self-report bias. Furthermore, we note that not storing images prevented objective validation of findings, interobserver reliability assessment, or concordance with gold-standard criteria. These aspects are now clearly recognized as factors that constrain the strength of our conclusions regarding feasibility and quality of POCUS implementation.

Comment 3:

“In addition, the manuscript does not provide sufficient information on the development and validation of the data collection forms. It is unclear whether the instruments underwent a systematic process of construction (e.g., based on literature review, expert consultation) and whether any procedures to establish evidence of content validity were performed. Without such information, it is difficult to assess whether the forms adequately captured the intended constructs (e.g., diagnostic hypotheses, management changes, confidence levels, time spent, and image quality). The absence of reported validity evidence represents an important limitation, as the accuracy and interpretability of the findings rely heavily on the adequacy of these instruments.”

We thank the reviewer for this valuable observation. In the revised manuscript, we clarified in the Methods that the data collection form was collaboratively developed by the authors, informed by prior studies in internal medicine/hospital medicine POCUS, and tailored to the specific objectives of this study. We also make explicit that the instrument was not formally validated and that no psychometric procedures (e.g., content validity assessment, inter-rater reliability testing) were performed.

Furthermore, we revised the Discussion to explicitly acknowledge this limitation. We highlight that, because the form was author-developed and not validated, the accuracy and interpretability of the constructs captured (diagnostic hypotheses, management changes, confidence levels, exam duration, and image quality) may be constrained. This limitation is now clearly stated alongside other methodological caveats.

Comment 4:

“In addition, it is unclear whether the POCUS training and subsequent examinations followed a standardized protocol, such as the RUSH protocol. The data collection form included the following examination categories: cardiac, pulmonary, abdominal, inferior vena cava, femoral and popliteal veins, bladder, and ‘other’. The RUSH protocol encompasses cardiac function and contractility assessment, evaluation of intravascular volume status via the inferior vena cava, identification of free fluid in the abdomen, lung assessment for pneumothorax or pulmonary embolism, and lower limb DVT screening. Given this overlap, it should be explicitly stated whether the investigators adopted the RUSH protocol in its entirety, used a modified version, or employed another structured approach. If the RUSH protocol was not used, the authors should clearly describe which anatomical regions and clinical targets were prioritized in both training and clinical application."

We thank the reviewer for raising this point. In the revised Methods, we clarified that neither the educational session nor the subsequent clinical examinations followed a standardized protocol such as RUSH. Examinations were performed entirely at the discretion of each physician, guided by the specific clinical question in each encounter.

The educational session provided a broad, introductory overview of POCUS for hospitalists, covering lungs/pleura, heart, abdomen, inferior vena cava, femoral/popliteal veins, and bladder, as well as basic equipment handling and image optimization. This was intended as an orientation, not as training in a fixed algorithm. The overlap between our data collection categories and components of the RUSH protocol reflects the fact that these organ systems are commonly relevant in hospital medicine POCUS, rather than adoption of RUSH or any other structured pathway.

Comment 5:

“The manuscript states that responses to open-ended questions were kept in their original format, but it does not describe the procedures adopted for their analysis. It remains unclear whether these responses were analyzed qualitatively (e.g., through thematic or content analysis), coded systematically by independent reviewers, or simply presented as anecdotal remarks. Without a clear description of the analytic approach, the contribution of these data to the study’s findings is difficult to assess. The authors should specify the method used to analyze the open-ended responses, including whether any coding framework, inter-rater reliability, or software tools were applied. If the responses were not systematically analyzed, this should be acknowledged as a limitation.”

We thank the reviewer for this important observation. In the revised manuscript, we clarified in the Methods that open-ended responses and relevant closed-ended items were transcribed into a structured Excel sheet and coded by the principal investigator for each form. Binary variables were created to capture changes in diagnostic hypotheses, diagnostic confidence, addition or exclusion of alternative diagnoses, incidental findings, and management changes. For diagnostic confidence, responses were recoded into three categories (Likert 1–2, 3, and 4–5) to define shifts across confidence levels. These derived variables were analyzed descriptively as categorical data. Open-text responses were also reviewed to extract brief contextual information; however, no formal qualitative analysis was performed, and no independent coding, inter-rater reliability procedures, or software-assisted thematic analysis were applied.

We also revised the Discussion to explicitly acknowledge this as a limitation. Specifically, we note that open-ended responses were categorized by a single investigator without independent review, coding framework validation, or reliability assessment, which may have introduced subjective bias. This caveat is now clearly presented alongside the other methodological limitations to aid readers in interpreting the findings.

Comment 6:

“The sample size rationale estimates a single post-POCUS proportion with 95% confidence and 10% margin of error, assuming p=0.20, yielding ≈62 forms. This is acceptable for estimating a single proportion. However, because multiple forms were submitted by the same hospitalists, observations are clustered. The calculation should therefore include a design-effect inflation based on the average forms per clinician and an assumed ICC. Using 5.5 forms/physician and plausible ICCs (0.05–0.10) increases the requirement to approximately 76–90 forms. The 104 forms collected likely satisfy this, but the manuscript should explicitly report the clustering adjustment (DEFF and ICC). In addition, the finite-population correction used should reflect the finite number of eligible encounters in the unit rather than the number of internists in the state; otherwise, FPC should be omitted.”

We thank the reviewer for this important observation. We agree that multiple forms submitted by the same physician introduce clustering, requiring adjustment. In the revised manuscript, we now explicitly report this. Because it is not possible to determine the finite number of eligible encounters within the Transitional Care Unit, we omitted the finite-population correction, as recommended by the reviewer, and applied the single-proportion formula assuming an infinite population.

In addition, we incorporated the reviewer’s recommendation to include a design-effect adjustment based on the average number of forms per physician and an assumed intraclass correlation coefficient (ICC). Since there are no prior studies to inform a precise ICC value in this context, we opted for a conservative estimate of ICC = 0.10. Using the observed average of 5.5 forms per hospitalist in our dataset, this adjustment increased the required sample size from 62 to approximately 90 forms. Our final dataset of 104 forms exceeded this threshold, thus satisfying the adjusted requirement.

Comment 7:

“The study demonstrates positive trends but does so with methodological fragility. The results should be framed more cautiously, emphasizing feasibility and potential utility rather than efficacy. Stronger study designs (e.g., randomized controlled trials or controlled before-and-after studies with validated outcome measures and objective performance data) would be necessary to substantiate these findings”

We fully agree and have revised the entire manuscript to adopt a more cautious framing. The revised text emphasizes feasibility and perceived influence on clinical reasoning. We now state that stronger study designs with validated protocols and competency assessment are required to confirm these findings.

Final response to reviewers

In addition to the revisions made in direct response to the reviewers’ comments, we implemented a few further improvements to enhance clarity and readability.

First, we edited the following sentence to improve the fluency of the text:

“Upon study completion, participants completed a survey (Fig 1) assessing their satisfaction with POCUS training, its usefulness and relevance in clinical practice, confidence in using the method, and any challenges encountered with the equipment (S3 Appendix C)” to: “Upon study completion, participants filled out a survey (Fig 1) assessing their satisfaction with POCUS training, its usefulness and relevance in clinical practice, confidence in performing POCUS, and any equipment-related challenges (S3 Appendix C).”

Second, because exam-time data did not follow a normal distribution according to the Shapiro–Wilk test (as described in the Methods), we replaced the mean with the median and interquartile range for the per-patient time: “The total time spent using POCUS was 935 minutes, with a median of 10 minutes per patient [5.00; 10.00].”

Third, to facilitate interpretation, we added Table 2 — POCUS Influence on Diagnostic Reasoning and De

Attachment

Submitted filename: Response_to_Reviewers_auresp_2.docx

pone.0338202.s006.docx (75.4KB, docx)

Decision Letter 2

Biswabandhu Jana

19 Nov 2025

Assessing the clinical relevance of point-of-care ultrasound for hospitalists: influence on clinical reasoning and decision-making

PONE-D-25-23495R2

Dear Dr. Dias Souza,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

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Kind regards,

Biswabandhu Jana, Phd

Academic Editor

PLOS ONE

Additional Editor Comments (optional):

Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

Reviewer #5: All comments have been addressed

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2. Is the manuscript technically sound, and do the data support the conclusions??>

Reviewer #5: Yes

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3. Has the statistical analysis been performed appropriately and rigorously? -->?>

Reviewer #5: Yes

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4. Have the authors made all data underlying the findings in their manuscript fully available??>

The PLOS Data policy

Reviewer #5: Yes

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5. Is the manuscript presented in an intelligible fashion and written in standard English??>

Reviewer #5: Yes

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Reviewer #5: The manuscript presents a well-structured and technically sound study. The objectives are clearly defined, and the methodology is appropriate for the problem being addressed. The authors provide a solid theoretical background, and the proposed approach is supported with adequate analysis and relevant comparisons. The results are presented in a clear and organized manner, demonstrating the effectiveness of the proposed method. Overall, the contribution is meaningful, and the technical content meets the standards expected for publication.

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Reviewer #5: No

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Acceptance letter

Biswabandhu Jana

PONE-D-25-23495R2

PLOS One

Dear Dr. Dias Souza,

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Associated Data

    This section collects any data citations, data availability statements, or supplementary materials included in this article.

    Supplementary Materials

    S1 Appendix. A. Form on participants’ personal data.

    Questionnaire completed by participants before the study. It collects demographic data, academic background, previous experience with point-of-care ultrasound (POCUS), and self-assessed confidence in performing and interpreting POCUS examinations.

    (DOCX)

    pone.0338202.s001.docx (26.1KB, docx)
    S2 Appendix. B. Form on POCUS clinical use.

    Structured form used by participants after each POCUS application during clinical practice. It documents the clinical question, diagnostic hypothesis, confidence levels before and after POCUS, selected ultrasound modalities, image quality, findings, and subsequent changes in diagnosis or management.

    (DOCX)

    pone.0338202.s002.docx (32.8KB, docx)
    S3 Appendix. C. Form on participants’ impressions and satisfaction survey.

    Survey assessing participants’ satisfaction with the POCUS course, perceived usefulness in clinical practice, importance of formal training, confidence levels after training, future interest in using POCUS, and perceived barriers to its implementation in the clinical setting.

    (DOCX)

    pone.0338202.s003.docx (55.2KB, docx)
    Attachment

    Submitted filename: Response to Reviewers.docx

    pone.0338202.s004.docx (32.7KB, docx)
    Attachment

    Submitted filename: PlosOne_Report.docx

    pone.0338202.s005.docx (15.7KB, docx)
    Attachment

    Submitted filename: Response_to_Reviewers_auresp_2.docx

    pone.0338202.s006.docx (75.4KB, docx)

    Data Availability Statement

    All relevant data are within the paper and its Supporting Information files.


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