TABLE 5.
Nucleotide differences between blaTEM-80 and other related blaTEM sequences
| bla gene (promoter)c | Nucleotidea (amino acidb) position in:
|
Reference | |||||
|---|---|---|---|---|---|---|---|
| Promoter region
|
Coding region
|
||||||
| 32 | 162 | 317 (39) | 407 (69) | 581 (127) | 1022 (276) | ||
| blaTEM-1A (P3) | C | G | C (Gln) | A (Met) | A (Ile) | A (Asn) | 39 |
| blaTEM-80 (Pa/Pb) | T | C (Leu) | G (Val) | G (Asp) | This study | ||
| blaTEM-127V (P3) | G (Val) | This study | |||||
| blaTEM-33 (P3) | C (Leu) | 38 | |||||
| blaTEM-35 (P3)d | A (Lys) | C (Leu) | G (Asp) | 44 | |||
| blaTEM-81 (Pa/Pb) | T | C (Leu) | G (Val) | 24 | |||
| blaTEM-84 (P4) | T | G (Asp) | 24 | ||||
a
Nucleotide positions are numbered as described by Sutcliffe (39) in comparison with the sequence of blaTEM-1A. Only nucleotide positions located in the coding region which lead to an amino acid substitution are indicated.
b
Amino acid numbering is according to the ABL numbering system of Ambler et al. (1).
c
Promoters are numbered as described by Leflon-Guibout et al. (23).
d
The promoter sequence of the blaTEM-35 gene used in this study was controlled by sequencing.