FIG. 2.
Identification of the transcriptional start site for blaCTX-M-17 in strain BM4493 by primer extension analysis. (A) Lane 1, primer elongation product obtained with oligodeoxynucleotide PE and 50 μg of total RNA of BM4493 (arrow); lanes T, G, C, and A, results of sequencing reactions performed with pIP843 DNA as a template and the PE primer. (B) Sequence from nt 2676 to 2950 (the numbering is that for the sequence of the strain with GenBank accession no. AY033516); +1, transcriptional start site for blaCTX-M-17 in BM4493, indicated by an arrow; the −35 and −10 promoter sequences upstream from the transcriptional start site are underlined with a thin line; the right terminal repeat of the ISEcp1-like element is underlined with a thick line; the ATG start codon of blaCTX-M-17 is boxed; and the ribosome-binding site (rbs) is underlined with dots. The location of the PE primer is indicated with an arrow at the bottom.