FIG. 2.
Gel electrophoresis of the amplified katG fragment and the products of its digestion by HapII. Lanes: 1, 2, 3, and 6, products obtained by HapII digestion of katG of INHr strains with mutated katG (315ACC); 4, 5, and 7, products obtained by HapII digestion of katG of strains not harboring the katG AGC→ACC mutation at codon 315; 8, artificially mixed digests of PCR products from both wild-type and ACC alleles of katG codon 315; 9, undigested amplified 200-bp katG fragment; M, 100-bp DNA ladder (Amersham Pharmacia Biotech).