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Molecular mass determination of native-form FabH using size exclusion chromatography. Log molecular mass versus elution volume/void volume ratio (Ve/Vo) is shown. The molecular mass standards used were β-amylase (200 kDa), alcohol dehydrogenase (150 kDa), bovine serum albumin (66 kDa), carbonic anhydrase (29 kDa), and cytochrome c (12.4 kDa). Vo was determined using blue dextran (2,000 kDa). The saFabH mass was measured at approximately 67 kDa.
