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Copper gradient BHI agar plate containing between 0 mM (bottom of the plate) and 28 mM (top of the plate) copper sulfate. (A) Lanes 1 and 3 contains copper-resistant strains, whereas lanes 2, 4, 5, 6, and 7 all contain copper susceptible strains. (B) Lanes 1, 3, and 5 contain copper-resistant donor strains, whereas lanes 2, 4, and 6 contain their respective transconjugants, and lane 7 contains the recipient strain (BM4105RF).
