Skip to main content
. 2003 Dec 11;23(1):54–65. doi: 10.1038/sj.emboj.7600012

Figure 4.

Figure 4

Isomerization results in SU dissociation. (A) Preparation of Mo-MLV with partially or completely isomerized SU–TM disulphide-bond. Mo-MLV was dialysed against TN, incubated as indicated, lysed in the presence of NEM, subjected to IP with anti-MLV pAb and analysed by nonreducing SDS–PAGE. (B,C) Isomerization results in dissociation of SU. Mo-MLV with partially (B) or completely (C) isomerized SU–TM disulphide-bond was subjected to floatation centrifugation. Fractions were immunoprecipitated and analysed by nonreducing SDS–PAGE. Note that four times less of loading zone (54%) has been analysed as compared to other fractions. (D) Conformational changes follow isomerization. Mo-MLV were incubated for 1–3 h at 37°C in TN with or without NEM, immunoprecipitated with pAb or mAb 500 and analysed by nonreducing SDS–PAGE.