Figure 7.

mCRMP1s are correctly localized in the cell and interact with both PlexA1 and CRMP2. (A) The subcellular localization of overexpressed mCRMP1s was examined in either transfected COS7 cells (upper row) or HSV-infected E8 chick DRG explants (lower row). All mCRMP1s showed a similar localization to overexpressed WT CRMP1. (B) HEK293T cells were transfected with either vector, V5-tagged WT CRMP1, or a V5-mCRMP1, and either Myc-tagged PlexA1 or CRMP2. Lysates were then immunoprecipitated with anti-V5 antibody and blotted with anti-Myc or anti-V5. Both Myc-PlexA1 and Myc-CRMP2 were able to co-immunoprecipitate with WT CRMP1 and all four mCRMP1s.