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. 2003 Dec 18;23(1):9–22. doi: 10.1038/sj.emboj.7600021

Figure 8.

Figure 8

mCRMP1(49–56) reduces neurite outgrowth in DRG neurons. (A) Neurite outgrowth from dissociated E8 chick DRG neurons infected with HSV-WT CRMP1 or HSV-mCRMP1(49–56) is visualized by anti-V5 immunoflourescence. Cells were cultured with or without 100 nM Sema3A. (B) Total neurite length/cell (mean±s.e.m.) was measured for dissociated E8 chick DRG neurons infected with HSV-EGFP, or V5-tagged HSV-WT CRMP1 or HSV-mCRMP1(49–56). Neurons were cultured with or without 100 nM Sema3A. mCRMP1(49–56) significantly reduced outgrowth compared to EGFP or WT CRMP1 (**P<0.005); Sema3A reduced outgrowth only in EGFP and WT CRMP-1 cultures (*P<0.05).