Table 1.
Double and triple mutant analyses of sur-7 and other genes for their roles in vulval induction
| Genotype | %M UV (n) | Average #VPC induced (n) |
|---|---|---|
| let-60(n1046) | 76 (128) | 4.62 (27) |
| let-60(n1046);sur-7(ku119) | 2 (634) | 3.03 (38) |
| let-60(kuIs12) | 86 (22) | 4.68 (22) |
| let-60(kuIs12);sur-7(ku119) | 17 (35) | 3.21(35) |
| lin-15(n765) | 91 (47) | 5.37 (51) |
| lin-15(n765) sur-7(ku119) | 41 (49) | 3.36 (37) |
| lin-31(n301) | 90 (49) | 3.81(20) |
| lin-31(n301);sur-7(ku119) | 95 (65) | 3.96 (20) |
| lin-1(ar147) | 100 (>100) | 5.91 (21) |
| lin-1(ar147);sur-7(ku119) | 100 (>100) | 5.85 (27) |
| Hsp-dRaf(gf) | 35 (60) | 3.84 (30) |
| Hsp-dRaf(gf);sur-7(ku119) | 35 (62) | 3.63 (28) |
| Hsp-dRaf(gf)a | 47 (30) | 3.57 (30) |
| Hsp-dRaf(gf);sur-6(ku123)a | 47 (17) | 3.75 (17) |
| Hsp-dRaf(gf);sur-8(ku167)b | ND | 3.75 (28) |
| Hsp-dRaf(gf);ksr-1(ku68)a | 46 (28) | 3.51(28) |
| Hsp-dRaf(gf);mpk-1(ku1)b | ND | 2.97 (28) |
| lin-45(gf) | 77 (334) | 4.44 (20) |
| lin-45(gf);sur-7(ku119) | 2 (435) | 3.06 (20) |
| lin-45(gf);sur-8(ku167) | 70 (246) | 3.81 (25) |
| Sur-6(ku123) lin-45(gf) | 0 (334) | 3.00 (23) |
| lin-45(gf);ksr-1(ku68) | 1 (98) | 3.00 (25) |
| Mek-2(ku114);lin-45(gf) | 0 (104) | 3.00 (25) |
| Hsp-mpk-1(gf) hsp-Dmek(gf) | 97 (240) | ND |
| Hsp-mpk-1(gf) hsp-Dmek(gf);sur-7(ku119) | 95 (273) | ND |
| Hsp-mpk-1(gf) hsp-Dmek(gf);sur-2(lf)c | 0 (25) | ND |
| Rol-4(sc8); let-60(n1046) | 86 (217) | ND |
| Par-1(b274);let-60(n1046) | 100 (112) | ND |
| Sur-6(ku123);let-60(n1046) | 6 (240) | ND |
| Sur-6(ku123);par-1(b274);let-60(gf) | 65 (48) | ND |
| let-60(n1046);par-1(b274);sur-7(ku119) | 94 (54) | ND |
| Sur-8(ku167) let-60(n1046) | 4 (333) | ND |
| Sur-8(ku167) let-60(n1046);par-1(b274) | 65 (111) | ND |
| let-60(n1046);ksr-1(ku68) | 0 (>100) | ND |
| let-60(n1046);par-1(b274);ksr-1(ku68) | 13 (23) | ND |
| Mpk-1(ku1);let-60(n1046) | 0 (>100) | ND |
|
Mpk-1(ku1);let-60(n1046);par-1(b274) |
2 (41) |
ND |
| %Muv, percentage of worms with a Multivulva phenotype examined under a dissecting scope. #VPC induced, number of vulval precursor cells induced to vulval cells examined under Nomarski optics on a compound microscope. (n), number of animals examined. ND, not determined. kuIs12 is an integrated array carrying multiple copies of wild-type let-60. For lin-15(n765) experiments, animals were grown at 18.2°C. hsp-dRaf(gf) is described in Materials and methods and Sieburth et al, (1999). lin-45(gf) is described in the text and Materials and methods. The hsp-mek/mpk-1(gf) strain has an integrated array containing gain-of-function mutant genes of both mpk-1 and d-mek (Lackner and Kim, 1998) (see Materials and methods for assay conditions. par-1 was marked with rol-4 in all lines tested. sur-8, sur-6 and ksr-1 were marked with dpy-20, unc-29 and lon-2, respectively. sur-7(ku119) and mek-2(ku114) homozygotes were identified by sequence analysis. Induction data were not collected for par-1 experiments in order to monitor embryonic phenotype to score for homozygous par-1(lf)). | ||
| a Sieburth et al (1999). | ||
| b Sieburth and Han (1998). | ||
| c Lackner and Kim (1996). | ||