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. 2003 Dec 18;23(1):100–110. doi: 10.1038/sj.emboj.7600033

Figure 4.

Figure 4

Association of Tube, MyD88, and Toll. (A) S2 cells were cotransfected with three different constructs: (1) V5-tagged Toll10B (Toll C781Y mutant); (2) vector DNA or V5-tagged MyD88; and (3) myc-tagged Tube. MyD88 and Tube mutants are as indicated. Immunoprecipitation was conducted with preimmune serum or anti-Tube serum. (Endogenous Tube may contribute to the residual coimmunoprecipitation of MyD88 and Toll10B in lanes 10, 12, and 13.) (B) Wild-type Toll or Toll10B was cotransfected into S2 cells with the listed combinations of empty vector, MyD88, and Tube. Anti-MyD88 or anti-Tube immunoprecipitation was then carried out as indicated. Toll and Tube protein in immunoprecipitates (upper) or cell lysates (bottom) were detected with anti-V5 and anti-myc antibodies, respectively. MyD88 protein was detected with anti-V5 antibody in immunoprecipitates and with anti-MyD88 antiserum in cell lysates. (C) Wild-type or a Tube binding-defective (D163K) mutant of MyD88 were assayed for their association with Toll10B as described in (B).