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. 2003 Dec 18;23(1):127–137. doi: 10.1038/sj.emboj.7600035

Figure 2.

Figure 2

Snf5p and histone acetyltransferase activities associate with the SUC2 promoter. (A) The crosslinking of Snf5p, Gcn5p, and Esa1p to the SUC2 UAS DNA was analyzed by ChIP in raffinose-induced wild-type (BLY1) cells using polyclonal antibodies against each protein and the levels (IP efficiencies) presented as fold over preinduction levels (upper panel). ChIP experiments using anti-HA or anti-Esa1p antibodies were performed in HA-ESA1 (BLY431) and ESA1 (BLY1) cells that were glucose repressed (time 0) or derepressed for 5 min. The SUC2 UAS and the promoter sequences of two ribosomal genes were amplified by PCR from the precipitated (IP) and input DNAs and stained with ethidium bromide after separation on 8% polyacrylamide gels (lower panel). (B) The acetylation of histone H3 at SUC2 UAS in wild-type (BLY1) and gcn5Δ (BLY417) cells was compared by ChIP using an antibody against diacetylated H3 (K9 and K14). The levels of acetylation (IP efficiencies) were shown relative to wild-type preinduction levels. (C) The levels of acetylation of H4 at SUC2 UAS in wild-type (BLY1) and esa1-Δ414 (BLY457) cells were compared by ChIP as in (B) using an antibody against tetra-acetylated H4 (K5, K8, K12, and K16).