Figure 3.

SUC2 promoter-specific association of chromatin remodelers or remodeling activities. (A) Crosslinking of Snf5p to the different SUC2 sequences (shown schematically below (C)) was determined by measuring [α-³²P]-dCTP-labeled multiplex PCR products of the precipitated and input DNAs from the same experiment for Figure 2A. IP efficiencies of SUC2 sequences were shown relative to that of a subtelomeric sequence (Vogelauer et al, 2000). The distribution pattern of Snf5p, 5 min following induction, is shown. The distances are relative to the translational start site. The binding of Snf5p to SUC2 following gene induction is under-represented for the sequences tested, as the levels of Snf5p crosslinking to the subtelomeric sequence following SUC2 induction also increased slightly. The distribution of H3 (B) and H4 (C, upper panel) acetylation at SUC2 was determined and presented as in (A). The distribution of Esa1p (C, lower panel) was similarly assayed from anti-HA ChIP experiments, except that IP efficiencies were shown in arbitrary units for comparison between HA-tagged and untagged (no tag) strains.