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Kinetic parameters obtained for unbinding from MT of monomeric rat kinesin (rK340) and dimeric rat kinesin (rK430) in the presence of ATP or ADP
| Parameter | rK340 |
rK430 |
||
|---|---|---|---|---|
| ATP | ADP | ATP | ADP | |
| kmax (s−1) | 73.6±1.7 | 40.5±1.1 | 42.4±2.7 | 18.6±0.6 |
| Khalf (μM) | 63.1±7.9 | 109.0±15.7 | 138.4±35.4 | 133.6±15.5 |
| kon (s−1) | 3.8±1.2 | 1.5±0.6 | 1.7±0.8 | 1.3±0.2 |
| K1k+2 (μM−1 s−1) | 1.17 | 0.37 | 0.31 | 0.14 |
| KI (μM) | 432±30 | 383±40 | 477±33 | 394±43 |
