Table 2.
Influence of phosphate on MT-activated ATPase and on the unbinding rates of monomeric rat kinesin (rK340) and dimeric rat kinesin (rK430)
| Effect of phosphate on MT-activated ATPase | rK340 |
rK430 |
||
|---|---|---|---|---|
| kcat (s−1) | K(0.5)MT (μM) | kcat (s−1) | K(0.5)MT (μM) | |
| Control | 28.83±2.09 | 3.87±0.86 | 47.85±3.07 | 0.71±0.20 |
| +40 mM KCl | 11.09±1.22 | 11.10±2.59 | 47.11±3.83 | 13.67±1.99 |
| +20 mM KPi | 16.01±3.81 | 9.75±6.23 | 42.84±2.82 | 3.43±0.72 |
| | ||||
| Effect of phosphate on unbinding rates | rK340 |
rK430 |
||
| |
ATP kmax (s−1) |
ADP kmax (s−1) |
ATP kmax (s−1) |
ADP kmax (s−1) |
| Control | 50.58±1.21 | 25.78±1.51 | 33.32±1.51 | 11.30±0.17 |
| +40 mM KCl | 49.36±0.77 | 23.19±0.65 | 19.04±0.48 | 9.56±0.21 |
| +20 mM KPi | 40.34±0.41 | 17.96±1.20 | 13.31±2.58 | 3.19±0.37 |