TABLE 2.

Transformation of S. pneumoniae CP1000 with PCR-amplified DNA from BM4478 and CIP5641T

Sequence in donor DNA (amino acid)a	Frequency of resistant recipient on rifampin at 10 μg/ml	Sequence in resistant recipient DNA (amino acid)
520-bp product
No DNA	1.2 × 10−6	TCA (Ser522)
TCA (Ser522)	1.3 × 10−6	TCA (Ser522)
TTA (Leu522)	3.1 × 10−3	TTA (Leu522)
609-bp product
No DNA	1.1 × 10−6	CAA (Gln722)
CAA (Gln722)	1.2 × 10−6	CAA (Gln722)
CAC (His722)	1.1 × 10−6	CAA (Gln722)

^aAmino acid positions are from E. coli coordinates.