Skip to main content
. 2003 Dec 18;23(2):302–311. doi: 10.1038/sj.emboj.7600036

Figure 4.

Figure 4

Ptc1 and Pbs2 associate in an Nbp2-dependent manner. Binding of HA-Ptc1 to GST-Pbs2 and GST was examined in the wild-type (JD52) and nbp2Δ (JHM17) strains. The strains carried pHA-PTC1 and pGST-PBS2 or the empty vector p(EG)KT, which expressed GST alone. GST-Pbs2 was isolated from yeast extracts using glutathione-sepharose, and the precipitated material was examined by SDS–PAGE and immunoblotting with anti-GST and anti-HA antibodies as described in Materials and methods. The amount of HA-Ptc1 in the lysates is also shown.