Figure 3.

STS induces CsA-suppressible and ER-independent apoptosis in primary myoblasts. (A) Western blot analysis of control and Apaf1^−/− myoblasts treated with vehicle (con), TG or STS with Bip antibodies. α-Tubulin was used as a loading control. (B) Levels of Bip relative to α-tubulin were determined by densitometry analysis of the Western blot in (A). TG but not STS induces Bip expression. (C) Confocal images of cytochrome c (green), mitotracker (red) and DAPI (blue) in untreated cultures or following 1 h treatment with STS alone or STS plus CsA. (D) AnnexinV analysis of indicated cultures treated with TG or STS either alone or together with CsA.