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. 2004 Jan 8;23(2):322–332. doi: 10.1038/sj.emboj.7600044

Figure 6.

Figure 6

Impaired translocation of TRAF2 into the insoluble fraction in HRS lymphoma cells coincides with failure to activate JNK. (A) Impaired translocation of TRAF2 into insoluble membrane rafts in HRS cells. HRS lymphoma (L-428) and anaplastic large cell lymphoma (SU-DHL-1) cells were treated with or without TNFα (20 ng/ml) for the indicated time points, and soluble and insoluble fractions were extracted. Protein samples were then subjected to immunoblot analysis using anti-TRAF2, anti-RIP1 and anti-Src antibodies. (B) Constitutive IκB degradation and impaired JNK activation in response to TNFα treatment in HRS cells. L-428 and SU-DHL-1 cells were treated with or without TNFα (20 ng/ml) for the indicated time points, and proteins were extracted with TNE buffer. Protein samples were then subjected to immunoblot analysis with anti-phospho-JNK and anti-IκB antibodies. The same membranes were stripped and blotted with anti-α-tubulin and anti-JNK antibodies as a loading control and for the detection of the level of JNK expression.