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. 2002 May;46(5):1402–1409. doi: 10.1128/AAC.46.5.1402-1409.2002

FIG. 1.

FIG. 1.

FIG. 1.

Mapping of integrons of S. marcescens SCH909. (a) General structures of integrons 1, 2, and 3 found in S. marcescens SCH909. The arrows show the direction of transcription. (b) PCR amplification from SCH909. The PCR products were separated by electrophoresis through 1.0% agarose. Lanes 1 and 14, 1-kb DNA ladder; lanes 2 to 13, SCH909 with the following primers: lane 2, Sulpro3 and 3′-CS; lane 3, Sulpro3 and dfrA1; lane 4, Sulpro3 and ant(3")-Ia; lane 5, Sulpro3 and ant(3")-Ii; lane 6, Sulpro3 and aac(6′)-Ib; lane 7, Sulpro3 and ant(2")-Ia; lane 8, Sulpro3 and aac(3)-Ia; lane 9, aac(3)-Iacooh and 3′-CS; lane 10, ant(3")-Iacooh and 3′-CS; lane 11, aac(3)-Iacooh and ant(3")-Ia; lane12, ant(2")-Iacooh and aac(6′)-Ib; and lane 13, tnpR and BLATEM. Primer Sulpro3 is a rightward primer in the 5′ conserved segment. Primers with gene names are leftward primers near the beginning of their respective cassettes. Primers with “cooh” are rightward primers near the end of their respective cassettes. Primer 3′-CS is a leftward primer in the 3′ conserved sequence. These primers are used to determine gene order in integrons (14).