TABLE 2.

Comparisons of ANP cytotoxicities to their interactions with DNA pol α, δ, and ɛ∗

ANP	ANPppKi/dNTPKmh			% fince, i			CC50f
	pol α	pol δ	pol ɛ*g	pol α	pol δ	pol ɛ*g	MT4j (μM)	CEMk (μM)	F-PBMCk (μM)	hRPTECl (μM)	HELm (μg/ml)
PMEGa	0.03	ND	0.004r	6.4	40.2	5.9h	11	≥0.8	0.23	ND	2.5
PMEDAPb	0.3	0.1	1.3r	2.6	20.5	4.2h	18	ND	ND	ND	40
(S)-HPMPAc	2.3	0.3	0.1r	7.6	8.7	18.4r	52; 26	ND	ND	ND	20
PMEAd	1.2	0.6	0.9r	0.7	4.4	2.0r	67	64	24	500	100
(R)-PMPA	10.2	10.2	15.6r	0.11	0.03	0.08r	>300	≥500	165	>2,000	ND

^aPMEG, 9-[(2-phosphonomethoxy)ethyl]guanine.

^bPMEDAP, 9-[(2-phosphomethoxy)ethyl]-2,6-diaminopurine.

^c(S)-HPMPA, (S)-9-[(3-hydroxy-2-phosphonomethoxy)propyl]adenine.

^dPMEA, 9-[(2-phosphonomethoxy)ethyl]adenine.

^ePercent f_inc = 100 × (^ANPppV_max × ^dNTPK_m)/(^dNTPV_max × ^ANPppK_m) (see reference 8).

^fCC₅₀, 50% cytotoxic concentration; ND, not determined.

^gh, human DNA polymerases; r, rat DNA polymerases.

^hSee reference 21.

ⁱSee references 5, 6, and 19.

^jSee references 1 and 26.

^kSee reference 1.

^lSee reference 11.

^mSee reference 2.