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. Author manuscript; available in PMC: 2025 Dec 21.
Published in final edited form as: Immunity. 2025 Oct 2;58(10):2472–2488.e9. doi: 10.1016/j.immuni.2025.09.008

Figure 1. Ablation of Tcf7+22kb limits IL-2 and IL-12-dependent downregulation of Tcf7 in vitro.

Figure 1.

(A) Analysis of ChIP (GEO: GSE58075, GSE85172, GSE79339) and ATAC (GEO: GSE88987) data of the Tcf7 locus in CD8+ T cells.

(B) Experimental approach corresponding to (C)–(E).

(C and D) Relative Tcf7 transcript levels normalized to Hprt1 in WT (closed circles) or Tcf7Δ+22kb (open circles) CD8+ T cells stimulated for 3 days (C) and subsequently rested for 3 days in 100 U/mL IL-2 or 10 ng/mL IL-15 (D). n = 2 in 2 independent experiments. Data are shown as mean ± SD. Unpaired t tests.

(E) Representative TCF-1 protein staining of WT (black) and Tcf7Δ+22kb (red) CD8+ T cells at indicated time points, representative of two experiments conducted in duplicate. Solid histograms represent post-stimulation conditions, and open histograms represent naive CD8+ T cells.

(F) Quantitation of the ratio of stimulated to naive TCF-1 MFI. Data are pooled from two experiments conducted in duplicate, represented as mean ± SD. Two-way ANOVA and Sidak’s multiple comparison test.

See also Figure S1.