Figure 4. scRNA-seq of WT and Tcf7^Δ+22kb CD8⁺ T cells in acute infection.

(A) UMAP plot of gp33-apecific CD8⁺ T cells sorted from two WT and two Tcf7^Δ+22kb mice 8 dpi with LCMV-Arm. Colored by cluster identity.

(B) WT and Tcf7^Δ+22kb cells displayed separately on UMAP projection.

(C) Stacked bar plot of frequency of each cluster in each replicate of gp33-specific CD8⁺ T cells.

(D) Heatmap of genes differentially expressed between clusters.

(E) Heatmap of genes differentially expressed in WT and Tcf7^Δ+22kb cells within each cluster.

(F) Feature plots of selected genes.

(G) Expression of GZMB, CD127, and CD62L analyzed by flow cytometry in gp33-specific CD8⁺ T cells from spleens of WT and Tcf7^Δ+22kb mice 8 dpi with LCMV-Arm. Data represent two experiments shown as mean ± SD and analyzed by t test. (n = 3 WT and 4 Tcf7^Δ+22kb mice).

(H) Volcano plot of bulk RNA-seq results from WT/Cd19 RNP and Tcf7^Δ+22kb/Tcf7^+17kb RNP P14 cells sorted as KLRG1⁺ on 8 dpi with LCMV-Arm WT/Cd19 RNP and Tcf7^+17kb RNP (n = 3). Cutoffs by adjusted false discovery rate (FDR) < 0.01 and fold-change > 2.

(I) Heatmap of selected receptors and TFs in RNA-seq of KLRG1⁺ WT/Cd19 RNP and Tcf7^Δ+22kb/Tcf7^+17kb RNP P14 cells of 8 dpi LCMV-Arm.

See also Figure S3.