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Two extra copies of the Pre6/α4 subunit in each pre9Δ proteasome. (A) Purified 20S proteasomes analyzed by gradient SDS–PAGE and Coomassie blue staining. (B) Average ratio of amino-acid levels in the indicated species (all are 20S proteasome subunits) from panel (A) in pre9Δ relative to wild-type proteasomes. (C) Partial suppression of pre9Δ by increased dosage of PRE6. Cells were spotted in 10-fold serial dilutions on minimal medium (SD) or SD containing 1 μg/ml canavanine.
