Skip to main content
. 2004 Jan 29;23(3):552–563. doi: 10.1038/sj.emboj.7600067

Figure 6.

Figure 6

Effects of SB431542 on osteoblastic differentiation of hMSCs. (A) hMSCs were differentiated into osteoblasts by OS medium in combination with or without DMSO or SB431542. The left panel shows ALP activity at day 7, and the right panel demonstrates calcium deposition on matrix as determined by von Kossa staining at day 14 after induction. (B) hMSCs were induced to differentiate into osteoblasts by BMP-4 (50 ng/ml) in serum-free media containing ITS supplements, 0.05 mM ascorbic acid-2-phosphate, and 10 mM β-glycerophosphate in combination with or without DMSO or SB431542. ALP activity was measured on day 7 (left panel) and von Kossa staining was performed on day 14 after induction (right panel). (C, D) Quantitative RT–PCR for human ALP gene (ALPL) (C) and BSP gene (IBSP) (D) was performed as described in Figure 2. hMSCs were treated with or without BMP-4 (50 ng/ml) in serum-free media containing ITS supplements with or without DMSO or SB431542 as described in panel B.