TABLE 2.
Effect of Ionic Amphiphile Biovector systems on the L. donovani LV9/BALB/c mouse model compared to AmBisomea
| Drug formulation | Treatment dose (mg of AmB/kg) | Stauber count (mean ± SEM) (108)b | % Parasite inhibition | Mouse weight (g) at autopsy (mean ± SEM) |
|---|---|---|---|---|
| ABV1/AmB | 0.25 | 2.55 ± 0.29∗ | 57.62 | 19.86 ± 0.56 |
| ABV1/AmB | 5 | 0.036 ± 0.011∗ | 99.39 | 19.92 ± 0.35 |
| ABV2/AmB | 0.25 | 3.33 ± 0.37∗ | 44.55 | 20.10 ± 0.39 |
| ABV2/AmB | 5 | 0.019 ± 0.0011∗ | 99.69 | 18.40 ± 2.23 |
| AmBisome | 0.25 | 2.15 ± 0.15∗ | 64.19 | 20.24 ± 0.74 |
| AmBisome | 5 | 0.015 ± 0.0013∗ | 99.77 | 20.20 ± 0.39 |
| Control | 5.94 ± 0.35 | 0 | 19.98 ± 0.63 |
There were 10 mice per batch, and i.v. treatments were given in a single dose.
∗, values are significantly different from those of the control (P < 0.001). At the dose of 5 mg/kg, the values obtained after the AmBisome, ABV1/AmB, and ABV2/AmB treatments were significantly lower than those of the control (P < 0.001), the values obtained after the AmBisome treatment were significantly lower than those obtained after the ABV1/AmB (P < 0.01) and ABV2/AmB (P < 0.03) treatments, and the values obtained after the ABV1/AmB and ABV2/AmB treatments were not significantly different. At the dose of 0.25 mg/kg, the values obtained after the AmBisome, ABV1/AmB, and ABV2/AmB treatments were significantly lower than those of the control (P < 0.001), the values obtained after the AmBisome treatment were significantly lower than those obtained after the ABV2/AmB treatment (P < 0.02), and the values obtained after the AmBisome and ABV1/AmB treatments were not significantly different.