Time-kill curves of S. aureus ATCC 9144 in control suspensions (♦) and after treatment with TTO (A), 1,8-cineole (B), terpinen-4-ol (C), or α-terpineol (D) at one-half the MICs (▵), the MICs (•), and two times the MICs (□). The MICs of TTO, terpinen-4-ol, and α-terpineol were 0.25% (vol/vol); and that of 1,8-cineole was 0.5%. The organisms were suspended in PBS-T. Each symbol indicates the means ± SEs for at least three replicates. The lower detection threshold was 103 CFU/ml.