TABLE 2.

Hyphal damage produced by amphotericin B or its lipid formulations alone and in combination with human PMNs against A. fumigatus^a

Compound	% Hyphal damage
	PMNs alone	Compound alone	PMNs + compound
DAMB	28.2 ± 8.0c	26.7 ± 8.0c	45.7 ± 7.0c,d
ABLC	28.2 ± 8.0c	49.1 ± 5.7b	60.7 ± 5.4b,c,d
LAMB	28.2 ± 8.0c	44.4 ± 7.3b	61.3 ± 4.5b,c,d

^aA 200-μl suspension of 1.5 × 10⁴ conidia in yeast nitrogen broth> was incubated in flat-bottom cell culture microplates at 32°C for 18 to 20 h. The yeast nitrogen broth> was then replaced by Hanks balanced salt solution, antifungal drugs, and PMNs at a 5:1 effector cell-to-target cell ratio. After incubation at 37°C in 5% CO₂ for 2 h and cell lysis> by washing with H₂O three times and shaking at room temperature for 5 min, 150 μl of phosphate-buffered saline containing 0.25 mg of XTT per ml and 40 μg coenzyme Q per ml was added. After incubation for an additional 60 min, 100 μl was transferred and read in a spectrophotometer (450 nm). Percent hyphal damage was calculated as (1 − X/C) × 100, where X and C are the optical densities of test wells and control wells with hyphae only, respectively. The results are shown as means ± SEMs of percent hyphal damage for seven duplicate experiments for each antifungal compound.

^bP < 0.05.

^cP < 0.01.

^dComparisons of antifungal compounds or PMNs and their combination were analyzed.