TABLE 1.
Bacterial strains and plasmids used in this studya
| Strains and vectors | Description | Reference or source |
|---|---|---|
| E. coli strains | ||
| AS19 | Permeable mutant of E. coli B strain | 37 |
| AS19-RrmA− | AS19 rrmA::kan, Kanr | This study |
| DH10B | F−mcrA Δ(mrr-hsdRMS-mcrBC) φ80d lacZΔM15 ΔlacX74 deoR recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λrpsL nupG | Life Technologies |
| JC7623 | F−thr-1 ara-14 leu-6 proA2 lacY1 sbcC201 tsx-33 galK2 sbcB15 his-4 recC22 recB21 rpsL31 xyl-5 mtl-1 argE3 thi-1 | E. coli Genetic Stock Center |
| Vectors | ||
| Bacteriophage P1 | Wild type | Laboratory collection |
| Plasmids | ||
| pMAK705 | Temperature-sensitive pSC101 replicon; Cmr | 18 |
| pSD184 | Derivative of pACYC184, Tcr; Kanr | This study |
| pSD57c | ermN/tlrD cloned into pSD184; Tcr | This study |
| pSDdiv | ermE cloned into pSD184; Tcr | This study |
| pSD707 | rrmA::kan fragment cloned into pMAK705; Cmr Kanr | This study |
a
Antibiotic abbreviations: Kan, kanamycin; Cm, chloramphenicol; Tc, tetracycline.