FIG. 4.
Sensitivity of GIV mutant Env to inhibition by T-20. One thousand infectious units of HIV-1 pseudotyped with mutant or wt Envs derived from patient 30-1 was used to infect cultures of JC53BL cells containing 0, 0.04, 0.2, 1, 5, and 25 μg of T-20 per ml. Two days later, virus entry was measured by analyzing the indicator cells for lucif activity. Virus infectivity was calculated by dividing the mean lucif activity value at each of the drug concentrations by the mean value of the control (0 mg). Resistance to T-20 is depicted by plotting relative infectivity on the y axis against T-20 concentration on the x axis. (A) HIV-1/SG3Δenv was pseudotyped with the G36D mutant Envs 57 and 2, the V38A mutant Envs 18 and 21, and wt Envs (wt-d0 and wt-d14). (B) Sequence analysis of the HR2 and V3 Env domains for the wt (d0-wt), no. 2 (d14-02), and no. 57 (d14-57) env clones. (C) HIV-1/SG3Δenv stocks were pseudotyped with G36D mutant Env 57, the 32H/36D double mutant Envs (32H, 36D), the 32R/36D double mutant Env (32R, 36D), and the wt Env (wt-d0). The results depicted in panels A and B were highly reproducible and are representative of three independent experiments.