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Gyrase-DNA binding assay. Gel retardation of 1 nmol of radiolabeled 140-bp DNA by increasing amounts of A2B2wt (A), A2B2426 (B), and A2B2447 (C) in the absence of drug and presence of excess GyrA. Apparent binding constants (Kdapps) were determined from phosphorimager analysis and were found to be 7.8 μM (A2B2wt) and 24.3 μM (A2B2426).
