TABLE 3.
Distribution of MICs of PGE 9262932, PGE 9509924, and comparator fluoroquinolones for 69 isolates of S. pneumoniae with no mutations (wild type) in the four QRDRs or with a single mutation or combinations of mutations within the QRDRs of gyrA and parC
| Amino acid alterations | No. of isolates | Quinolone | No. of isolates for which the MIC (μg/ml) was:
|
|||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GyrA | ParC | ≤0.008 | 0.015 | 0.03 | 0.06 | 0.12 | 0.25 | 0.5 | 1 | 2 | 4 | 8 | 16 | ≥32 | ||
| None | None | 8a | PGE 9262932 | 3 | 3 | 2 | ||||||||||
| PGE 9509924 | 4 | 2 | 2 | |||||||||||||
| Moxifloxacin | 1 | 3 | 2 | 2 | ||||||||||||
| Gatifloxacin | 1 | 5 | 2 | |||||||||||||
| Levofloxacin | 1 | 5 | 2 | |||||||||||||
| Ciprofloxacin | 1 | 4 | 2 | 1 | ||||||||||||
| None | None | 21b | PGE 9262932 | 11 | 5 | 5 | ||||||||||
| PGE 9509924 | 1 | 11 | 5 | 3 | 1 | |||||||||||
| Moxifloxacin | 1 | 14 | 6 | |||||||||||||
| Gatifloxacin | 1 | 2 | 14 | 4 | ||||||||||||
| Levofloxacin | 1 | 5 | 12 | 3 | ||||||||||||
| Ciprofloxacin | 1 | 5 | 10 | 5 | ||||||||||||
| None | Lys137Asn | 6c | PGE 9262932 | 3 | 1 | 2 | ||||||||||
| PGE 9509924 | 1 | 2 | 2 | 1 | ||||||||||||
| Moxifloxacin | 6 | |||||||||||||||
| Gatifloxacin | 6 | |||||||||||||||
| Levofloxacin | 4 | 2 | ||||||||||||||
| Ciprofloxacin | 1 | 3 | 2 | |||||||||||||
| None | Ser79Phe | 2d | PGE 9262932 | 1 | 1 | |||||||||||
| PGE 9509924 | 1 | 1 | ||||||||||||||
| Moxifloxacin | 2 | |||||||||||||||
| Gatifloxacin | 1 | 1 | ||||||||||||||
| Levofloxacin | 1 | 1 | ||||||||||||||
| Ciprofloxacin | 1 | 1 | ||||||||||||||
| Ser81Tyr | None | 3e | PGE 9262932 | 3 | ||||||||||||
| PGE 9509924 | 2 | 1 | ||||||||||||||
| Moxifloxacin | 1 | 2 | ||||||||||||||
| Gatifloxacin | 1 | 1 | 1 | |||||||||||||
| Levofloxacin | 3 | |||||||||||||||
| Ciprofloxacin | 1 | 1 | 1 | |||||||||||||
| Ser81Phe | None | 2d | PGE 9262932 | 1 | 1 | |||||||||||
| PGE 9509924 | 1 | 1 | ||||||||||||||
| Moxifloxacin | 1 | 1 | ||||||||||||||
| Gatifloxacin | 1 | 1 | ||||||||||||||
| Levofloxacin | 1 | 1 | ||||||||||||||
| Ciprofloxacin | 1 | 1 | ||||||||||||||
| Ser81Phe | Ser79Phe | 16f | PGE 9262932 | 2 | 7 | 6 | 1 | |||||||||
| PGE 9509924 | 1 | 4 | 6 | 5 | ||||||||||||
| Moxifloxacin | 6 | 9 | 1 | |||||||||||||
| Gatifloxacin | 4 | 8 | 4 | |||||||||||||
| Levofloxacin | 8 | 4 | 4 | |||||||||||||
| Ciprofloxacin | 4 | 6 | 6 | |||||||||||||
| Ser81Phe | Ser79Phe, Lys137Asn | 4a | PGE 9262932 | 2 | 2 | |||||||||||
| PGE 9509924 | 2 | 2 | ||||||||||||||
| Moxifloxacin | 3 | 1 | ||||||||||||||
| Gatifloxacin | 2 | 2 | ||||||||||||||
| Levofloxacin | 2 | 2 | ||||||||||||||
| Ciprofloxacin | 2 | 2 | ||||||||||||||
| Ser81Phe | Asp83Asn, Lys137Asn | 2a | PGE 9262932 | 1 | 1 | |||||||||||
| PGE 9509924 | 1 | 1 | ||||||||||||||
| Moxifloxacin | 1 | 1 | ||||||||||||||
| Gatifloxacin | 2 | |||||||||||||||
| Levofloxacin | 2 | |||||||||||||||
| Ciprofloxacin | 2 | |||||||||||||||
All isolates had the wild-type sequences GyrB and ParE.
Twelve isolates had the wild-type sequence at GyrB and the Ile460Val alteration in ParE, one isolate had the Arg541Lys alteration in GyrB and the Ile460Val alteration in ParE, one isolate had the Ala639Gln alteration in GyrB and the Ile460Val alteration in ParE, five isolates had the wild-type sequence at GyrB and the Lys466Met alteration in ParE, one isolate had the Ala639Gln alteration in GyrB and the wild-type sequence at ParE, and one isolate had the Ala538Ser alteration in GyrB and the wild-type sequence at ParE.
Three isolates had wild-type sequences at GyrB and ParE, two isolates had the wild-type sequence at GyrB and the Ile460Val alteration in ParE, and the isolate had the Ala639Gln alteration in GyrB and the wild-type sequence at ParE.
One isolate had wild-type sequences at GyrB and ParE, and one isolate had the wild-type sequence at GyrB and the Ile460Val alteration in ParE.
Two isolates had wild-type sequences at GyrB and ParE, and one isolate had the wild-type sequence at GyrB and the Ile460Val alteration in ParE.
Seven isolates had wild-type sequences at GyrB and ParE, five isolates had the wild-type sequences at GyrB and the Ile460Val alteration in ParE, one isolate had the wild-type sequence at GyrB and the Pro454Ser alteration in at ParE, one isolate had the Ala639Gln alteration in GyrB and the wild-type sequence at ParE, one isolate had the Arg541Lys alteration in GyrB and the Ile460Val alteration in ParE, and one isolate had the Ala639Gln alteration in GyrB and the Ile460Val alteration in ParE.