FIG. 5.

Proteins released by FLC-resistant strain AIDS 126 of C. albicans in the culture medium in the absence and in the presence of FLC. (a) SDS-10% polyacrylamide gel stained by use of the Silver Stain Plus kit; (b) transblotting onto nitrocellulose paper and reaction with antienolase immune serum; (c) transblotting onto nitrocellulose paper and reaction with ConA-digoxigenin. In all cases the gels were loaded with 1 μg of protein. Lanes 1, control supernatants of FLC-untreated cells; lanes 2, supernatants of FLC-treated cells. Molecular mass standards are expressed on the right of each gel (in kilodaltons). For other technical details, see Materials and Methods.