TABLE 1.

P. furiosus ORFs that lack homologs in the P. woesei genome based on DNA microarray and PCR analysesa

ORFb	A	B	C	InterPRO annotation		ORFb	A	B	C	InterPRO annotation
PF0025	*	*	*	Glutamine amidotransferase II		PF0735				No InterPRO entry
PF0026	*	*	*	DNA polymerase, beta region		PF0737				No InterPRO entry
PF0029				No InterPRO entry		PF0738				S-Adenosylmethionine binding protein
PF0030				No InterPRO entry		PF0739				Bacterial regulator, AsnC/Lrp
PF0031				PLP enzyme, beta subunit		PF0740			*	Copper-transporting ATPase
PF0032				No InterPRO entry		PF0742			*	Ferritin and Dps
PF0034		*		No InterPRO entry		PF0743				Coenzyme A binding domain
PF0035		*		ABC transporter		PF0744			*	ABC transporter
PF0037				ARM repeat fold		PF0758	*	*		HEPN, nucleotide-binding
PF0038	*	*		Beta-lactamase-like		PF0760	*		*	Transposase, IS605 OrfB
PF0041	*	*	*	Elongator protein 3/MiaB/NifB		PF0762	*	*		No InterPRO entry
PF0151	*			Glycoside hydrolase, family 5		PF0763				No InterPRO entry
PF0152		*	*	Protein of unknown function		PF0764	*	*	*	Archaeal ATPase
PF0154	*	*	*	Glutamine amidotransferase II		PF0765		*		6-Phosphogluconate DH, C terminal
PF0365				Protein of unknown function		PF0766		*		Oxidoreductase, N terminal
PF0366	*	*	*	No InterPRO entry		PF0767		*		Deg T aminotransferase
PF0685				Protein of unknown function		PF0768		*		Transferase hexapeptide repeat
PF0687				Transcription factor TFIIB		PF0769		*		Glycosyl transferase, family 2
PF0689				Prismane-like		PF0770		*		UTP-glucose-1-Pi transferase
PF0691		*		Lambda repressor-like		PF0772	*			DNA polymerase, beta-like
PF0692		*		Prismane		PF0773				No InterPRO entry
PF0693				SirA-like		PF0775				No InterPRO entry
PF0694				Flavodoxin/NO synthase		PF0776			*	No InterPRO entry
PF0695		*		Protein of unknown function		PF0777				No InterPRO entry
PF0697				Sugar transporter superfamily		PF0781		*	*	Nucleotide binding protein, PINc
PF0698				No InterPRO entry		PF0783		*		No InterPRO entry
PF0701	*	*		No InterPRO entry		PF0790				No InterPRO entry
PF0702	*	*		Peptidase M24		PF0791				Glycosyl transferase, group 1
PF0703				No InterPRO entry		PF0792				No InterPRO entry
PF0704				Protein of unknown function		PF0794				UDP-N-Ac gluc.-2-epimerasec
PF0705				Cytochrome c biogenesis		PF0795				Glycosyl transferase, group 1
PF0706				Protein of unknown function		PF0796				Transferase hexapeptide repeat
PF0707				4-Oxalocrotonate tautomerase		PF0797				No InterPRO entry
PF0708				Extrusion protein MatE		PF0798				Glycosyl transferase, family 2
PF0712				No InterPRO entry		PF1337	*	*		TENA/THI-4 protein
PF0713		*		Winged helix DNA binding		PF1339				Protein of unknown function
PF0715				Nitroreductase		PF1340				Protein of unknown function
PF0716				6-Phosphogluconate dehydrogenase		PF1737				No InterPRO entry
PF0717				S-Adenosylmethionine binding motif		PF1738				Ribokinase
PF0718				No InterPRO entry		PF1339				Protein of unknown function
PF0719				No InterPRO entry		PF1740				Membrane transport component
PF0720				No InterPRO entry		PF1741				Membrane transport component
PF0721				NADPH: flavin mononucleotide reductase		PF1742	*			Glycosyl transferase, group 1
PF0722				Alkyl hydroperoxide reductase		PF1743	*			Protein of unknown function
PF0723				Iron permease FTR1		PF1744	*	*	*	ABC transporter
PF0725				Coenzyme A binding domain		PF1745	*	*		l-Fucose isomerase
PF0727				No InterPRO entry		PF1746				Glycoside transferase
PF0729				4Fe-4S ferredoxin		PF1747				Protein of unknown function
PF0730			*	No InterPRO entry		PF1748			*	Membrane transport component
PF0731				Ferritin-like		PF1749			*	Membrane transport component
PF0732				ABC transporter		PF1750	*	*	*	ABC transporter
PF0733				ABC transporter		PF1751	*	*		Thiamine ABC transporter
PF0734				No InterPRO entry

^aShading indicates potential ORF clusters (in which adjacent ORFs can be on the positive or negative strand). An asterisk indicates the presence of a homolog. See text for details.

^bFor each ORF, the presence of a homolog in P. horikoshii (A), P. abyssi (B), or T. kodakaraensis (C) is shown.

^cUDP-N-acetylglucosamine-4-epimerase.