TABLE 1.

Amino acid changes corresponding to the hlyD mutants sequenced and secretion phenotype (production or not of hemolytic haloes on blood agar plates)

HlyD mutation			HlyA secretion phenotype
HlyD mutant	Amino acid change	Codon change
HlyD7	K404→E	1210AAG→GAG	Null
HlyD8	V349→I	1045GTT→AAT	Conditionala
HlyD35	T85→I	A254CT→ATT	Conditional
HlyD45	V334→I	1000GTT→ATT	Conditional
HlyD54	L165→Q	C496TA→CAA	Conditional
HlyD55	D411→N	1231GAT→AAT	Null

^aMutants producing extremely small hemolytic haloes on LB blood agar plates but displaying clearly detectable haloes on minimal medium.