TABLE 2.
Oligonucleotide PCR primers and FISH probes
| Primer name | Oligonucleotide sequencea (5′-3′) | Reference | Morphotypes hybridized |
|---|---|---|---|
| 27F | AGAGTTTGATCCTGGCTCAG | 29 | |
| 1492R | GGTTACCTTGTTACGACTT | 29 | |
| 1423R Epulo probe | *TTGCGGTTAGGTCACTGACGTTGGGCCCCCT | 5 | All |
| C1 probe (198R) | *TTTGCCGTAGCCACCATGC | This study | C1 |
| C2 probe (198R) | *CTTTGCTATACACACCATGC | This study | C2 |
| J1 probe (198R)b | *ATGCGATAAAATTAGTGTATGC | This study | J1 |
| J2 probe (827R) | *ATCCTTAAATCCCGACACCT | This study | J2 |
| J group probe (1260R) | *CGTCACCGCTATGCTT | This study | J1 and J2 |
| 515F probe | *GTGCCAGCMGCCGCGG | 15 | None |
| Euk probe | *GGGCATCACGGACCTG | 15 | None |
a
5′ 6-carboxyfluorescein-modified primers are marked with an asterisk.
b
Weak hybridization signal was observed.