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Effects of exoA and nfo mutations on the wet-heat resistance of (A) wild-type and (B) α−β− spores. Spores of wild-type and α−β− strains were incubated at 90°C, and viability was determined as described in Materials and Methods. ▪, PS832 (wild type); ▴, PERM452 (exoA); •, PERM453 (nfo); ⧫, PERM454 (exoA nfo); □, PS356 (α−β−); ▵, PERM448 (α−β− exoA); ○, PERM449 (α−β− nfo); ◊, PERM450 (α−β− exoA nfo). The error bars are the standard deviations as described in Materials and Methods.
