TABLE 1.
Strains and plasmids used
| Strain or plasmid | Genotype or description | Source or reference |
|---|---|---|
| Strains | ||
| E. coli | ||
| XL10-Gold | Tetr Δ(mcrA)183 Δ(mcrCB-hsdSMR-mrr)173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac The [F′ proAB lacIqZDM15 Tn10 (Tetr) Tn5 (Kanr) Amy] | Stratagene, Cedar Creek, TX |
| PERM337 | E. coli XL10-Gold carrying plasmid pPERM337; Neor Tetr | This study |
| PERM374 | E. coli XL10-Gold carrying plasmid pPERM374; Neor Tetr | This study |
| B. subtilis | ||
| 168 | trpC2 | Laboratory stock |
| PS356 | ΔsspA ΔsspB α−β− | 17 |
| PS832 | Wild type; trp+ revertant of strain 168 | Laboratory stock |
| PS2488c | katA Cmr | 3 |
| PS2493c | ahpF Cmr | 3 |
| PS3672c | ΔyfhQ::spc Spcr | P. Setlow (27) |
| PS3673c | ΔmutM::tet Tetr | P. Setlow (27) |
| PS3677c | ΔmutM::tet ΔyfhQ::spc Spcr Tetr | (PS3672→PS3673)a(27) |
| PERM448 | ΔsspA ΔsspB ΔexoA::tet α−β− Tetr | (pPERM374→PS356)b |
| PERM449 | ΔsspA ΔsspB Δnfo::neo α−β− Neor | (pPERM337→PS356)b |
| PERM450 | ΔsspA ΔsspB ΔexoA::tet Δnfo::neo α−β− Neor Tetr | (pPERM337→PERM448)b |
| PERM452 | ΔexoA::tet Tetr | (pPERM374→PS832)b |
| PERM453 | Δnfo::neo Neor | (pPERM337→PS832)b |
| PERM454 | ΔexoA::tet Δnfo::neo Neor Tetr | (pPERM337→PERM452)b |
| PERM504 | exoA-lacZ trpC2 Cmr | (pPERM490→168)b |
| PERM527 | exoA-lacZ sigGΔ1 trpC2 Cmr | (pPERM490→WN118)b |
| WN118 | sigGΔ1 trpC2 | P. Setlow (39) |
| Plasmids | ||
| pDG1515 | Vector containing a Tetr cassette; Ampr Tetr | R. Yasbin (12) |
| pBEST501 | Vector containing a Neor cassette; Ampr Neor | R. Yasbin (16) |
| pJF751 | Integrational lacZ fusion vector; Cmr | W. L. Nicholson (10) |
| pPERM282 | pUC18 with 1.07-kb XbaI-BamHI PCR product containing nfo; Ampr | Laboratory stock |
| pPERM337 | 1.3-kb SmaI-SmaI fragment containing a Neor cassette inserted into the NaeI site of pPERM282; Ampr Neor | This study |
| pPERM359 | pDG1515 with 469-bp XbaI-BamHI PCR product containing the 5′ region of exoA; Ampr Tetr | This study |
| pPERM374 | pPERM359 with 537-bp EcoRI-HindIII PCR product containing the 3′ region of exoA; Ampr Tetr | This study |
| pPERM489 | pCR-Blunt II-TOPO with a 752-bp EcoRI-SmaI PCR fragment encompassing 434 bp upstream and 318 bp downstream of the exoA translational start codon; Neor | This study |
| pPERM490 | Translational exoA-lacZ fusion in pJF751; Ampr Cmr | This study |
a
Chromosomal DNA from the strain to the left of the arrow was used to transform the strain to the right.
b
Plasmid DNA from the strain to the left of the arrow was used to transform the strain to the right.
c
The background for this strain is PS832.