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Predicted RNA secondary structure of the rpoS ribosome-binding sequence. Mfold was used to predict the RNA secondary structure of a 48-nt region of the rpoS transcript: 24 nt directly preceding the rpoS start codon (labeled with an asterisk) extending to rpoS codon 8 (47). The bases of the extended Shine-Dalgarno sequence of rpoS are individually circled. Arrows indicate positions of directed mutations; the substituted nucleotides are also shown, as described in the text.
