FIG. 1.

Dendrogram of the main clusters of the DHA12 family. The tree consists of representatives from each cluster in the DAH12 family. Cluster A consists of drug and multidrug transporters from yeast and fungi. The rest of the clusters consist of sequences from different bacteria and archaea. The novel multidrug transporters (CGmdr, MSmdr, and HSmdr) form differentiated branches in the same cluster with VMAT (cluster C). The sources of the sequences in the dendrogram are as follows. Cluster A: UM05248—Ustilago maydis, CNBH0110—Cryptococcus neoformans, YALI0ACHR—Yarrowia lipolytica, Yhr048W—Saccharomyces cerevisiae, UM02490—Ustilago maydis, CyhR—Candida maltosa, and CaMDR1—Candida albicans. Cluster B: Bcr—Burkholderia mallei, LP_Flo—Legionella pneumophila, PP_Flo—Photobacterium damselae, CmlA—Pseudomonas aeruginosa, CflA—Coxiella burnetii, EmrD—Escherichia coli, MdfA—Escherichia coli, MDT—Yersinia pestis biovar Medievalis, and YjiO—Escherichia coli. Cluster C: rVMAT2—Rattus norvegicus, hVMAT2—Homo sapiens, hVMAT1—Homo sapiens, HMmdr—Haloarcula marismortui, HSmdr (YfmO2)—Halobacterium sp. NRC-1, CEmdr—Corynebacterium efficiens, CGmdr—Corynebacterium glutamicum, MSmdr—Mycobacterium smegmatis, and MAmdr—Mycobacterium avium. Cluster D: EF_MEP—Enterococcus faecium, Bmr—Bacillus subtilis, NorA—Bacillus cereus, TetA—Escherichia coli, TetG—Pasteurella multocida. Cluster E: CmlA—Pseudomonas aeruginosa, OpdE—Pseudomonas aeruginosa, LmrP—Lactococcus lactis.