TABLE 1.

Timeline of comparison of high and low doses of azithromycin with placebo in EOM due to NTHI^a

Day	Step
−3	Shave and examine superior bullae of chinchillas
−2	Inoculate middle ears with 10-50 CFU of H. influenzae
1	Access bullae; obtain MEF for culture; administer azithromycin at 30 or 120 mg/kg or placebo via orogastric intubation
2	Obtain blood by superior cranial sinus puncture (selected animals); administer azithromycin at 30 or 120 mg/kg or placebo via orogastric intubation; obtain blood by superior cranial sinus puncture for pharmacodynamic study (selected animals)
3	Obtain blood by superior cranial sinus puncture (selected animals); access bullae; obtain MEF for culture and azithromycin levels (intracellular and total); administer azithromycin at 30 or 120 mg/kg or placebo via orogastric intubation
4	Administer azithromycin at 30 or 120 mg/kg or placebo via orogastric intubation
5	Obtain blood by superior cranial sinus puncture (selected animals); access bullae; obtain MEF for culture azithromycin at 30 or 120 mg/kg or placebo via orogastric intubation
9	Access bullae; obtain MEF for culture and azithromycin level (intracellular and total)
11	Access bullae; obtain MEF for culture

^aFor details, see Materials and Methods.