FIG. 4.

Chemical synthesis of acyl-Cys-GlcN-Ins from a reaction of 250 μM Cys-GlcN-Ins with 1 mM of propionyl-CoA to produce prop-Cys-GlcN-Ins (○), of acetyl-CoA to produce MSH (▿) and of succinyl-CoA to produce succ-Cys-GlcN-Ins (□) in 50 mM HEPES, pH 7.4, containing 1 mM DTT at 30°C. Lines correspond to the second-order rates calculated from the rate constants given in the text. The data shown are representative of three independent experiments which produced similar results.