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Autoxidation of thiols in the presence of 50 mM HEPES, pH 7.3, containing 0.3 μM CuSO4. (A) ○, 200 μM MSH; •, 200 μM Cys-GlcN-Ins. (B) 100 μM MSH (○) plus 100 μM Cys-GlcN-Ins (•); total thiol (□). Three independent experiments all produced results essentially identical to the one shown.
