FIG. 6.
Dot blot analysis of lmo0422 transcripts from PSPAC-422E strains. Cultures of the Δlmo0422/PSPAC-422E and ΔsigC/PSPAC-422E were grown at 37°C to mid-logarithmic phase (OD600, ∼0.4), and RNA was extracted from cells harvested prior to or 30 min after addition of IPTG to a final concentration of 1 mM. As a control, RNA was also extracted from mid-logarithmic-phase cells of the Δlmo0422 strain 30 min after upshift of the culture to 48°C. Samples of 20 μg of RNA (each) were then spotted onto nylon, cross-linked by UV light, and then hybridized with a 32P-labeled PCR product derived from the lmo0422 coding region, which includes 180 bp of the 3′ end of sigC, the entire lmo0422 coding region, and 182 bp of the 5′ end of lmo0421.