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. 2005 Nov;187(21):7243–7253. doi: 10.1128/JB.187.21.7243-7253.2005

FIG. 7.

FIG. 7.

Ectopic expression of lmo0422 rescues the Δlmo0422 and ΔsigC thermal sensitivity phenotypes. Cells of the wild-type, Δlmo0422, ΔsigC, Δlmo0422/PSPAC-422E, and ΔsigC/PSPAC-422E strains were grown overnight in BHI, diluted 1:200 in fresh BHI, and grown to mid-logarithmic phase (OD600, ∼0.4). The cultures were then subdivided into several equal portions, and IPTG was then added at 0, 0.5 μM, 1 μM, 2.5 μM, and 5 μM concentrations to the cultures of the PSPAC-lmo0422E strains. After 30 min, samples of the cultures were removed and enumerated by serial dilution and plating. The remaining portions of the cultures were then transferred to 60°C. Samples were removed at various times after temperature upshift and enumerated by serial dilution and plating. (A) ○, 10403S; □, Δlmo0422; ⧫, Δlmo0422/PSPAC-422E; ▪, Δlmo0422/PSPAC-422E plus 0.5 μM IPTG; ▴, Δlmo0422/PSPAC-422E plus 1 μM IPTG; •, Δlmo0422/PSPAC-422E plus 2.5 μM IPTG. (B) ○, 10403S; □, ΔsigC; ⧫, ΔsigC/PSPAC-422E; ▴, ΔsigC/PSPAC-422E plus 1 μM IPTG; •, ΔsigC/PSPAC-422E plus 2.5 μM IPTG. Data shown are representative of three or more independent experiments.