TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant genotype and features | Reference or source |
|---|---|---|
| Strains | ||
| SM10 | E. coli conjugation donor; F−thi-1 thr-1 leuB6 recA tonA21 lacY1 supE44 (Muc+) λ−[RP4-2(Tc::Mu)] Kmr Tra+ | 52 |
| RM1602 | E. coli (dam recA negative) | W. Haldenwang |
| TOPO10 | E. coli host strain F−mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZ ΔM15 ΔlacX74 recA1 deoR araD139 Δ(ara-leu)7697 galU galK rpsL (Strr)endA1 nupG | Invitrogen |
| 10403S | L. monocytogenes wild type | 7; N. Freitag |
| ΔsigC | L. monocytogenes ΔsigC derivative of 10403S | This study |
| Δlmo0422 | L. monocytogenes Δlmo0422 derivative of 10403S | This study |
| Δlmo0421 | L. monocytogenes Δlmo0421 derivative of 10403S | This study |
| ΔsigC/PSPAC-422E | pPD422E integrated in strain ΔsigC | This study |
| Δlmo0422/PSPAC-422E | pPD422E integrated in strain Δlmo0422 | This study |
| Plasmids | ||
| pDEH21 | PSPAC vector | N. Freitag |
| pPD422E | PSPAC-controlled lmo0422 | This study |
| pPL2 | Site-specific integration vector | 38 |
| pSKV7 | Temperature-sensitive integration vector | 54 |
| pKSV7Δlmo0421 | Δlmo0421 allele in the pKSV7 vector | This study |
| pKSV7Δlmo0422 | Δlmo0422 allele in the pKSV7 vector | This study |
| pKSV7ΔsigC | ΔsigC allele in the pKSV7 vector | This study |
| pCR4-TOPO | Cloning vector | Invitrogen |