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Effect of synthetic indole alkaloids on the NO production by nonactivated (open bars) and IFN-γ-LPS-activated (hatched bars) J774.A1 macrophages. Supernatants were harvested 24 h after treatment, and nitrite concentration was estimated by Griess reaction. Data represent means with standard errors of the means (error bars) of two independent cultures done in triplicate. All compounds were tested at 10 μg/ml. Abbreviations: NA, untreated nonactivated cells; ACT, untreated activated cells.
