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Effect of synthetic indole alkaloids on IL-6 production by nonactivated (open bars) and IFN-γ-LPS-activated (hatched bars) J774.A1 macrophages. Supernatants were harvested 24 h after treatment, and IL-6 production was determined by ELISA. Results are from one representative experiment out of three, all done in triplicate. All compounds were tested at 10 μg/ml. Abbreviations: NA, untreated nonactivated cells; ACT, untreated activated cells; GLU, N-methylglucamine.
