FIG. 2.

HPLC profiles of reaction mixtures containing INH (2 mM), NAD⁺ (2 mM) (A) or DNAD⁺ (2 mM) (B), and Mn(III) pyrophosphate (4 mM; introduced in 10 consecutive additions of 400 μM each, every 2 min) in phosphate buffer (100 mM), pH 7.5. See Fig. 1 for the compound formula corresponding to each peak. Compounds 4 and 10 coelute and compound 4 is the minor component. ∗ and ∗∗, nicotinamide and nicotinic acid coming from decomposition of NAD⁺ and DNAD⁺, respectively. RRT, relative retention time (with INH as reference).