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Protection of InhA activity by NADH or decenoyl-CoA against INH-NAD(H) adducts. Column 1, control experiment in the absence of adducts with 5-min preincubation of InhA (column 1; 100%); columns 2 to 4, remaining InhA activities when InhA was preincubated with either 100 nM of the isolated pool of adducts (column 2; 22%), 100 μM NADH (column 3; 104%), or 100 μM decenoyl-CoA (column 4; 90.5%). Experiments were performed in duplicate (errors of < 7%).
